[Histonet] Re: Histonet Digest, Vol 61, Issue 25

Tyrone Genade tgenade <@t> gmail.com
Tue Dec 16 04:24:38 CST 2008

Hi all,

> Date: Mon, 15 Dec 2008 18:56:28 +0800
> From: " tf " <tifei <@t> foxmail.com>
> Subject: [Histonet] Silver staing for degenerating axons

> But I dont have the original pdf for the paper on Stain Technology in 1951 & 1954. Anyone can
> send me the pdf?


> Can anyone provide me a supressive procedure in staining degenerating axons using "common
> chemicals", just ammonia water, silver nitrate, citric acid, formalin/PFA, sodium hydroxide? I
> just can not find applicable one with our current facilities!

Have you read Sun et al (2002) Comparative analysis of an improved
thioflavin-S stain, Gallas Silver Stain, and immunohistochemistry for
neurofibrillay tangle demonstration of the same sections. J. Histochem
& Cytochem 50(4):463--472?

The reference for Gallays is: Gallays (1971) Silver staining of
Alzheimer's neurofibrillary changes by means of physical development.
Acta Morphol Acad Sci Hung 19:1--8.

Otherwise, you may want to look into FlouroJadeB. If all you are after
is visualizing degenerating axons, there are easier ways of going
about ot than silver staining.

Kind regards

Tyrone Genade
email: tgenade <@t> freeshell.org
tel: +27-84-632-1925 (c)
"For there is one God, and there is one mediator between God and
men, the man Christ Jesus, who gave himself as a ransom for all."
        1 Timothy 2:5-6

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