[Histonet] mouse dendritic cells

Gayle Callis gayle.callis <@t> bresnan.net
Fri Dec 12 17:22:17 CST 2008


We stain for murine dendritic cells very frequently.  CD11c (HL3 clone) is 
excellent from BD Biosciences using fresh snap frozen tissue with spleen as 
the positive control.   Fixation is with our beloved 25% ethanol/75% acetone 
for 5 min at RT then going directly to buffer from fixative.  The sections 
are air dried overnight before fixation.  Dec 205, NLDC 145 from Serotec 
works but we have superior results with the BD Pharm rat antiMouse CD11c HL3 

There are a whole series of new DC antibodies out there, used by our FACS 
tech, but I have not tried them on frozen sections yet.  I would still used 
the same tissue preparation and fixation if we did try them but also toss in 
cold acetone just for posteriety sake.  The CD11c we work with is 
biotinylated, which simplifies everything since we come back with 
Streptavidin Alexa dyes for single and double immunofluorescence work these 
days.  It is an Armenian hamster host IgG1.

Happy Holidays to you

Gayle Callis
Bozeman MT

----- Original Message ----- 
From: "Andrea Hooper" <anh2006 <@t> med.cornell.edu>
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, December 12, 2008 3:11 PM
Subject: [Histonet] mouse dendritic cells

> Is anyone staining for mouse dendritic cells either in paraffin or frozen 
> sections? If so, what markers/antibodies are you using?
> Thanks, ANDREA
> -- 
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