[Histonet] Re: Non-gyn cytology prep - amount processed?
RSRICHMOND <@t> aol.com
Thu Dec 11 20:20:33 CST 2008
Cathy Crumpton asks (the message seems to have gotten garbled in transmission):
>> Hi all, for those of you who also work with cytology - what is procedure for fluids that have a large volume? How much of the fluid do you process? - I am wanting to compare our polic instance this week where we received processed 100 mL of that after making sure it first cell blocks only contained fibrin, but the malignant cells. When we went back to the contai settled after 24 hours so we poured off the top part of t and only processed the gunk at the bottom. The second time t were malignant cells in the cell block. My pathologist is pushin for us to process 100% of the fluids, 100% of the time, saying what we cu get those<<
You certainly cannot process an entire large liquid specimen for
cytologic examination - many pleural fluid specimens are well over a
litre. There are a number of ways to prepare a cell block from such a
specimen - an important adjunct to the cytologic examination
examination of such fluids. A lot of laboratories have trouble
preparing cell blocks consistently.
I'd suggested getting a method from a cytopathology service that has
it working - and probably visiting the service. If your pathologist
thinks you can process 100% of a one litre specimen, he isn't going to
be of much help to you in getting a procedure working.
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