[Histonet] Re: Non-gyn cytology prep - amount processed?
bethcoxx <@t> gmail.com
Thu Dec 11 17:58:46 CST 2008
Your message came through a little garbled, but I think I get the jist
Standard procedure for large volume cytology fluid specimens is to mix
the specimen well, and then take off a 50 ml aliquot to centrifuge down
and make the slides and cell block. If the specimen is not mixed, you
are likely to miss the settled malignant cells. It is not prudent (or
recommended!) to centrifuge down the entire specimen. If a specimen
were extremely sparsely cellular, I might occasionally spin down two 50
I agree that if a specimen has set for a while and settled naturally,
pouring off the supernatent and using the concentrated precipitate for a
50 ml aliquat can be a nice bonus. BUT, there should have been
malignant cells in the WELL-MIXED original aliquot. What prep
procedures are you using for the slides?? ThinPrep processing can be
subject to problems with these kind of specimens because sometimes the
filter becomes 'clogged' with fibrin and protein, and the processor
thinks it has cells, but not much is there. ThinPrep may not be the
best choice for body fluid specimens.
A well done cell block should also have shown cells in it. If you are
simply scraping loose cells into a lens paper to process and try to
embed for a cell block, I'm not surprised if you lost the cells and only
recovered the more prominent fibrin. It's always a good idea to use
some method to actually hold (block!) the cells together - such as agar,
thrombin-prothrombin, or albumin clot.
Beth Cox, SCT/HT(ASCP)
Date: Thu, 11 Dec 2008 10:10:31 -0800
From: Cathy.Crumpton <@t> tuality.org
Subject: [Histonet] Non-gyn cytology prep - amount processed?
To: histonet <@t> lists.utsouthwestern.edu
<OF6CE74DE0.44DC7092-ON8825751C.0063D701-8825751C.0063D708 <@t> tuality.org>
Content-Type: text/plain; charset="ISO-8859-1"
Hi all, for those of you who also work with cytology - what is procedure for fluids that have a large volume? How much of the fluid do you process?
I am wanting to compare our polic instance this week where we received processed 100 mL of that after making sure it first cell blocks only contained fibrin, but the malignant cells. When we went back to the contai settled after 24 hours so we poured off the top part of t and only processed the gunk at the bottom. The second time t were malignant cells in the cell block. My pathologist is pushin for us to process 100% of the fluids, 100% of the time, saying what we
cu get those
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