[Histonet] Silly Question?
Tony Henwood
AnthonyH <@t> chw.edu.au
Thu Dec 11 16:18:05 CST 2008
Where is the evidence - or is this another mythical beast some of us
believe?
I don't believe it.
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Linda M
Watson
Sent: Friday, 12 December 2008 4:11 AM
To: Pat Flannery
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Silly Question?
Hi Pat,
Paraformaldehyde does not contain any additives and is considered more
"pure" than formaldehyde which often contains methanol which in some
cases is undesirable depending on the type of assay being conducted.
Linda
Pat Flannery wrote:
> Please humor me on this if it's obvious (to everyone but me): why do
> we use paraformaldehyde (which is so inconvenient to make up) rather
> than buffered formalin or just diluted formaldehyde itself?
>
> It seems that around here, some folks prefer paraformaldehyde (either
> 2% or 4%) and others use formalin, while some others stick to diluted
> formaldehyde (I see all 4 on labels for specimens submitted for
> histology). Is it mostly a matter of personal preference or where
> you were trained (i.e. force of habit) or is there a valid reason to
> use each solution (basically the same chemical once in solution,
> merely buffered or not)? The only answer I've gotten when I've asked
> is, "That's what we always use."
>
> Thanks.
>
> -Pat Flannery (not a "real" histologist - I just play one in the lab)
>
>
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