[Histonet] glutaraldehyde autofluoresence

ejschmid <@t> ucalgary.ca ejschmid <@t> ucalgary.ca
Thu Dec 11 15:50:13 CST 2008


My glutaraldehyde fixed mouse embryos fluoresce slightly reddish orange
under rhodamine. I have read on this website's archives that using a
solution of "bland amino acids" can help get rid of this. Does anyone have
a protocol to fix this problem or can someone explain to me how and why
this works?

Eric Schmidt

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