[Histonet] PCNA staining on paraffin

[anh2006 <@t> med.cornell.edu]

Second this, the DAKO clone works great.

-----Original Message-----
From: Jan Shivers <shive003 <@t> umn.edu>

Date: Thu, 11 Dec 2008 10:01:38 
To: Merced Leiker<leiker <@t> buffalo.edu>
Cc: histonet<histonet <@t> lists.utsouthwestern.edu>
Subject: Re: [Histonet] PCNA staining on paraffin


I use Dako's PCNA (cat. # M0789), mouse monoclonal, clone PC10, HIER with 
pressure cooker (Decloaker, 30" at 121C, 21psi) in Target Retrieval buffer, 
45 min in primary antibody (current dilution is 1:200, but that varies with 
each new lot), 30 min in goat anti-Ms EnVision+/HRP, 15 min in AEC.  I use 
an autostainer, but it can be done manually with the same incubation times 
at RT.

This works for me on many mammalian species.

Jan Shivers
UMN VetDiagLab
St. Paul, MN

----- Original Message ----- 
From: "Merced Leiker" <leiker <@t> buffalo.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, December 10, 2008 1:07 PM
Subject: [Histonet] PCNA staining on paraffin


> Does anyone have any suggestions for staining PCNA on paraffin? We are 
> using Santa Cruz's PCNA (FL261) SC-7907.
>
> We have already stained with it successfully on frozen sections, but it 
> does not appear to be working on paraffin. Thank you.
>
> Merced M Leiker
> Research Technician II
> 354 BRB (pkgs) / 140 Farber Hall (letters)
> School of Medicine and Biomedical Sciences
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>
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