[Histonet] divalent ions in fix

Rene J Buesa rjbuesa <@t> yahoo.com
Sat Dec 6 15:51:43 CST 2008

During time, besides the re-polymerization, methanal (= formaldehyde) turns into methanoic (acid) and the buffer salts prevent that acidification.
René J.

--- On Sat, 12/6/08, ejschmid <@t> ucalgary.ca <ejschmid <@t> ucalgary.ca> wrote:

From: ejschmid <@t> ucalgary.ca <ejschmid <@t> ucalgary.ca>
Subject: [Histonet] divalent ions in fix
To: histonet <@t> lists.utsouthwestern.edu
Date: Saturday, December 6, 2008, 3:32 PM


What is the function of divalent ions in fix?

It's been suggested to me that by using Dulbeccos PBS to make formaldehyde
fix from PFA will better preserve tissues, or at least better preserve

Eric Schmidt

University of Calgary
Medical Sciences

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