[Histonet] divalent ions in fix

[Rene J Buesa]

During time, besides the re-polymerization, methanal (= formaldehyde) turns into methanoic (acid) and the buffer salts prevent that acidification.
René J.

--- On Sat, 12/6/08, ejschmid <@t> ucalgary.ca <ejschmid <@t> ucalgary.ca> wrote:

From: ejschmid <@t> ucalgary.ca <ejschmid <@t> ucalgary.ca>
Subject: [Histonet] divalent ions in fix
To: histonet <@t> lists.utsouthwestern.edu
Date: Saturday, December 6, 2008, 3:32 PM

Hi,

What is the function of divalent ions in fix?

It's been suggested to me that by using Dulbeccos PBS to make formaldehyde
fix from PFA will better preserve tissues, or at least better preserve
proteins.


Eric Schmidt

University of Calgary
Medical Sciences




_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet