[Histonet] Bouin's fixation and paraffin or vibratome sectioning

ejschmid <@t> ucalgary.ca ejschmid <@t> ucalgary.ca
Fri Dec 5 11:30:02 CST 2008


Question about use of Bouin's fix on mid-gestational mouse embryos...We
 fix our ED 10-11 embryos in Bouin's and embed for sectioning, either
 paraffin or vibratome.

What we experience is that the tissues suffer quite a bit of damage, as
 the sections are often ripped. It sometimes seems to us that the Bouin's
 fixed tissue is really dry or even brittle. Do others have a similar

Eric Schmidt
University of Calgary
Medical Sciences

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