[Histonet] IHC on fresh frozen
anh2006 <@t> med.cornell.edu
Thu Dec 4 10:12:06 CST 2008
Try fixing for 5-10 min in PFA.
At 10:16 AM -0500 12/3/08, FU,DONGTAO wrote:
> Recently I did some IHC(chromagen methods) on mouse fresh frozen
>tissues, mainly using insulin antibody on pancreas. The image is
>much fuzzier compare to paraffin embedding tissue. And the staining
>also smeared to acinar cells which surround the islet.
> I airdried slide(>30min) and used a general acetone method(-20C
>5min) to fix the tissue before I did IHC.
> How can I get a relatively sharp staining on the fresh frozen
>tissue?Does anyone here have any experience on it? Any suggestions?
> Many thanks and have a nice day,
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