[Histonet] IHC on fresh frozen

Andrea Hooper anh2006 <@t> med.cornell.edu
Thu Dec 4 10:12:06 CST 2008

Try fixing for 5-10 min in PFA.

At 10:16 AM -0500 12/3/08, FU,DONGTAO wrote:
>Hi, all
>  Recently I did some IHC(chromagen methods) on mouse fresh frozen 
>tissues, mainly using insulin antibody on pancreas. The image is 
>much fuzzier compare to paraffin embedding tissue. And the staining 
>also smeared to acinar cells which surround the islet.
>  I airdried slide(>30min) and used a general acetone method(-20C 
>5min) to fix the tissue before I did IHC.
>  How can I get a relatively sharp staining on the fresh frozen 
>tissue?Does anyone here have any experience on it? Any suggestions?
>  Many thanks and have a nice day,
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu


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