[Histonet] IHC on paraformaldehyde-fixed

[Yaskovich, Ruth A (NIH/NIDCR) [E]]

I do it all the time. Yes your antigen-retrieval method will work. 
Ruth 
N.I.H.

-----Original Message-----
From: Jan Shivers [mailto:shive003 <@t> umn.edu] 
Sent: Wednesday, December 03, 2008 4:34 PM
To: histonet
Subject: [Histonet] IHC on paraformaldehyde-fixed

Has anyone ever done IHC on parafomaldehyde-fixed tissues, and if so,
how well did it work?  Will the same antigen-retrieval methods used with
formalin-fixed tissue be applicable?

I'm asking for an investigator, who already has his tissues fixed in
paraformaldehyde.

Jan Shivers
Senior Scientist
Pathology Teaching Program
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 <@t> umn.edu
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