[Histonet] Insulin/Glucagon double IHC

[Galina Deyneko]

Dear Colleagues,
I would like to ask your advices. I should establish double IHC staining on FFPE isolated mouse pancreatic islets for alpha and beta cells. I have very restricted amount of slides and I do not have possibility to test different antibodies and protocols. Could you please give me references what are the best antibodies for insulin and glucagon for mouse and for human (for the future study) Langerhans islets staining.I think for double staining it is better to use the antibodies from different species. If you could share with me the protocols, I mean some tips about  antigen retrieval, antibody dilution and incubation time, I will really appreciate it. As a secondary antibody i use the polymers from Biocare.
Thank you in advance.
Galina Deyneko.
Novartis, Cambridge, MA
617-871-7613