[Histonet] T Cell and B Cell Markers on Lymph Node Touch Preps

[Sandra Cheasty]

Hello,

            Does anyone have experience with performing Immunocytochemistry (CD3, CD20) on touch preps of canine and feline lymph nodes, particularly the following:

 

Fixation: What reagent(s), what temperature, how long, immediately or wait until air dried, storage post fixing

 

Antigen Retrieval: If formalin is part of the fixative, is there a general rule for HIER on ICC specimens that relates to HIER on paraffin sections of lymph nodes for CD3 and CD20?

 

Thank you so very much.

 

 

 

Sandra Cheasty

Histology & Necrcopsy Supervisor

UW-Madison 

School of Veterinary Medicine