[Histonet] DAB: old-fashioned style!

[Rene J Buesa]

6 mg of DAB dissolved in 15 mL of PBS adding 200 µL of 3% hydrogen peroxide. 
First dissolve with agitation half of the DAB, then add the rest of the DAB and at the end add the hydrogen peroxide. The hydrogen peroxide will end at a concentration of 0.04% There you have it!
René J.

--- On Fri, 8/29/08, Kim Merriam <kmerriam2003 <@t> yahoo.com> wrote:

From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
Subject: [Histonet] DAB: old-fashioned style!
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Date: Friday, August 29, 2008, 1:06 PM

Hi All,

It's been many moons since I had to make DAB by hand and I have lost my
recipe.  Can anyone let me know how to make DAB from powder and H2O2?

Thanks,
Kim
 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 



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