[Histonet] Hematoxylin ... STILL!!

[Lee & Peggy Wenk]

If the Weigert is for a Trichrome - skip it. The important part is muscle
red, collagen blue or green. On most trichromes, the black of Weigert
doesn't stick around anyway, and the nuclei are end up red. If you don't
tell them, they would probably never notice the lack of Weigert in a
trichrome. 

In place of VVG, how about an aldehyde fuchsin (using acetaldehyde instead
of paraldehyde if making your own), or the resorcin fuchsin stain (usually
available in kit form if you want it that way). Or the Orcein stain, also
known as Pinkus. All of these stain fine and coarse elastin, while VVG only
stains coarse elastin, so the pathologists just have to get used to seeing
MORE elastin than they are used to, and that the elastin will be violet,
blue/black or brown, not black. But hey, they have stained elastin.

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amos Brooks
Sent: Wednesday, August 27, 2008 7:36 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Hematoxylin ... STILL!!

Hi,
   OK In the absence of hematoxylin, the substitutes can hold people over
that run out of pre-made stuff. What are people doing for powder? Are there
any non-hematoxylin substitutes for Weigerts Iron hematoxylin? How about
Veerhoffs elastic stain? My order was placed with Sigma in April ... Back
ordered 'till Aug 15th ... still waiting ...

MONGO GETTING MAD ... GRR!

Amos
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet