[Histonet] (para)formaldehyde

[Monfils, Paul]

10% formalin and 4% paraformaldehyde are interchangeable for most purposes (in histology at least).  However there are a couple of minor differences.  First, commercial formaldehyde solution contains 37% to 38% formaldehyde.  Therefore diluting it 1:9 results in a solution containing 3.7% to 3.8% formaldehyde, while a 4% solution of paraformaldehyde in water contains a full 4% formaldehyde.  Secondly, commercial formaldehyde solution contains 10% to 15% methanol as a preservative.  Therefore diluting it 1:9 results in a solution containing 1.0% to 1.5% methanol.  This is not a problem for most histological applications, but it could be a problem in a procedure where sources of methylation have to be avoided.  4% paraformaldehyde solution contains no methanol - nothing but water and formaldehyde.