[Histonet] (para)formaldehyde (was: in situ question)

[Bernice Frederick]

Well said. Now if we could have the post-docs here at NU read and
understand.......A lot of our PI's are just as bad. I have issues when we
get a new post-doc from a lab and no one tells them anything about the
project they are taking over. We usually have to tell them. Just ask how
they want it oriented or grossed  and see the blank look on their faces. I
would think there should be some science background somewhere.
Bernice


Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
ECOGPCO-RL 
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rittman,
Barry R
Sent: Wednesday, August 27, 2008 9:24 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] (para)formaldehyde (was: in situ question)

While it is true that most people are "trainable" most post docs generally
do not have the time to put in to become proficient, and in any case we may
not have the time to train them. When they learn about lab techniques it is
generally only from the point of view of "what is the minimum that I need to
know as I only have a very limited time to do this". 
Histotechs in general are continually learning outside this narrow box.
Without this attitude work would become a never ending tedious task.

I think that what most people do when teaching post docs the basics of
tissue preparation is give them samples that will always work. This gives
them a false impression and they will often believe that histology is very
easy. It is only if given some of the challenging material that histotechs
have to cut that they will get a true appreciation of the level of skill
involved.
While may pathologists have a very good idea of the processes involved in
turning out sections, some do not and share the same opinion that many post
docs often have.
If I had my choice I would make it mandatory for all post docs and
pathologist to spend 6 months in a lab in order to experience the range of
problems that can occur. I have found that those post docs and pathologists
that have some experience in a histo lab are much more considerate of the
work carried out by histotechs and more realistic with their requests.
My opinion.
Barry

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, August 27, 2008 8:54 AM
To: 'Mikael Niku'; 'Tony Henwood'; histonet <@t> lists.utsouthwestern.edu; 'Geoff
McAuliffe'; Bernice Frederick
Subject: RE: [Histonet] (para)formaldehyde (was: in situ question)

Very good everything you said EXCEPT for "Post-doc are not tech and never
will be" because anybody and everybody that is willing to learn and pay
attention to what they do CAN and WILL become a good tech. 
Being a tech is NOT a genetic trait within the great scheme of evolution,
you know?
René J.

--- On Wed, 8/27/08, Bernice Frederick <b-frederick <@t> northwestern.edu> wrote:

From: Bernice Frederick <b-frederick <@t> northwestern.edu>
Subject: RE: [Histonet] (para)formaldehyde (was: in situ question)
To: "'Mikael Niku'" <mikael.niku <@t> helsinki.fi>, "'Tony Henwood'"
<AnthonyH <@t> chw.edu.au>, histonet <@t> lists.utsouthwestern.edu, "'Geoff
McAuliffe'" <mcauliff <@t> umdnj.edu>
Date: Wednesday, August 27, 2008, 8:39 AM

We receive tissue from researchers that was fixed in PFA. It goes into 10%
NBF once it hits the processor. Sort of becomes s moot point for us. A lot
of times it's a result of reading a VERY old paper or method (or so
we've
discovered)and they don't know any better. Post-docs are not techs and
never
will be.
Bernice


Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
Histology supervisor
ECOGPCO-RL 
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723




      
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