[Histonet] bone marrow decalcification

Mark Tarango marktarango <@t> gmail.com
Fri Aug 22 16:32:36 CDT 2008


Hi John,

Are you sure that the BM biopsies are completely fixed before going
into decal?  No pinkish or reddish-colored cores should be put into
decal.

I'd look into the fixation time before decalcification.  If you can
increase this, it might be all that you need to do.

Mark


On 8/22/08, Ryan, John P. <jryan <@t> sleh.com> wrote:
> What method do you utilize for the decalcification of bone marrow specimens that does not impair immunohistochemical staining? We have noted that our immunos (not surprisingly) stain less intensely on decalcified bone marrow than on non-decalcified tissue and would like to minimize this effect.
>
> John Ryan
> St. Luke's Episcopal Hospital
> 832-355-2643
> jryan <@t> sleh.com
>
>
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