[Histonet] Hyaluronidase

[Tony Henwood]

William,

Hyaluronidase, to my knowledge has not been successfully used to unmask
antigens like Proteinase K and trypsin.

It has been successfully used in Mesothelioma immunohistochemistry to
remove hyaluronic acid prior to immunostaining. Sometimes a background
staining with anti CEA antibodies can occur if there is an abundant of
hyaluronic acid present. Staining was cleaned-up after hyaluronidase
pre-treatment.


Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of William
Connor
Sent: Friday, 22 August 2008 7:14 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Hyaluronidase


Greetings,

 

How interchangeable are hyaluronidase and Proteinase K for an IHC
procedure?


 

If the protocol calls for hyaluronidase, had I better use it
specifically?

 

William P. Connor, HT(ASCP)

Senior Histology Technician

Histology Tech Services, Inc.

 

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