[Histonet] fibrin gel to paraffin

[Patsy Ruegg]

Betsy,
I would wrap them in paper (perm curl paper) and process as usual on a
tissue processor, I use a shorter than usual schedule for animal tissue and
things like this, 20 min in each change of alcohols, xylene and paraffins.
Embed on edge to get a cross section of all the layers.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Molinari,
Betsy
Sent: Thursday, August 21, 2008 5:44 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] fibrin gel to paraffin

Hi,

I have some "outgrowths" that are in a modified fibrin gel. They vary in
size from.1 to 1mm thick. The gel is porous and optically clear for the
most part. They have been fixed in 4% paraformaldehyde. They were done
in well plates or chamber slides.

 The researchers want them processed for paraffin. I have no experience
with this technique and would be grateful for any suggestions.

Thank you.

 

Betsy Molinari   HT(ASCP)

Texas Heart Institute 

Cardiovascular Pathology

6770 Bertner Ave.

MC 1-283

Houston, TX 77030

832-355-6524

832-355-6812 (fax)

 

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