[Histonet] electron microscopy for peroxidase activity

[Teresita Insausti]

Does anybody know the tetramethylbenzidine method 
for locating endogenous peroxidase activity using 
transmission electron microscopy? Could somebody help me, please?
The protocol that I found says: ..."Transfer the 
tissue to freshly prepared 0.5 mg/ml 
tetramethylbenzidine in phosphate buffer (pH 4). 
After 3, 5, 7 hr add 0.01 % freshly prepared 
H2O2. Leave overnight and wash the tissue in cacodylate buffer"...
1) Is the indicated H2O2 concentration (0.01 %) 
the final one after adding it to the buffer 
containing the tetramethylbenzidine or that of the H2O2 solution to be added?
2) If so, what is the volume to be added?
Thank you very much in advance
Teresita


Dr. Teresita Insausti
Institut de Recherche sur la Biologie de l'Insecte (IRBI)
Université François Rabelais - Parc Grandmont
37200 Tours
France

>e-mail: tere.insausti <@t> univ-tours.fr
>Phone: +33 (0)2 47 36 73 66
>Fax: +33 (0)2 47 36 69 66
>http://www.univ-tours.fr/irbi/