[Histonet] get back eosin stain?

[Andrea Grantham]

>

I see this a lot with cultured cells - even when processed and 
embedded in paraffin.

Andi Grantham


>Hello,
>
>I have a colleague with a staining problem. He has cells grown on polymeric
>scaffolds. He can not embedd in paraffin due to a mismatch in the processing
>and his scaffold material; he has thus embedded in OCT.
>
>This is his procedure:
>Fix cell seeded scaffold in formalin (these are delicate samples 
>thus, fixing before embedding)
>Cryoprotect in grade sucrose to 30% sucrose
>Embed in OCT
>Section at 6um
>General H&E staining-using 1 min for Hematox and 1.5 min for Eosin (following
>one of Richard Allan suggested protocols).
>
>Problem: there is no eosin staining. The cells are just purple, 
>there is no pink at all. We are not sure why this is. Is this caused 
>by fixation?
>
>Does someone have any suggestions on how he can get the classic H&E 
>stain look for these samples? He has tried fresh solutions as well 
>as running other tissue (unfixed) through. It doesn't seem to be the 
>solutions used.
>
>Any suggestions would be appreciated. Thank you
>Yak-Nam
>
>
>Reserach Associate
>CIMU
>University of Washington
>Box 355640
>Seattle, WA 98195
>
>Tel.:
>
>
>
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.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
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: (FAX:  520-626-2097)          (email:  algranth <@t> u.arizona.edu)       :
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