[Histonet] Freezing Haematoxylin

[Barnhart, Tammy]

Yep, if it freezes it's worthless.  From a North Dakota histotech.  We HAVE
to make our own.  Winter shipments of premaid hematoxylin always get too
cold and the staining is very erratic.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Rene J
Buesa
Sent: Friday, August 15, 2008 12:08 PM
To: louise renton; Histonet <@t> lists.utsouthwestern.edu; Weems, Joyce
Subject: RE: [Histonet] Freezing Haematoxylin


Ah, the things you learn when living in the "cold area" of the country!
René J.

--- On Fri, 8/15/08, Weems, Joyce <JWeems <@t> sjha.org> wrote:

From: Weems, Joyce <JWeems <@t> sjha.org>
Subject: RE: [Histonet] Freezing Haematoxylin
To: "louise renton" <louise.renton <@t> gmail.com>,
Histonet <@t> lists.utsouthwestern.edu
Date: Friday, August 15, 2008, 12:07 PM

I don't think so... If hematoxylin freezes in shipping, it crystallizes
and must be replaced. My 2 cents worth... 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of louise
renton
Sent: Friday, August 15, 2008 3:41 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Freezing Haematoxylin

Dear all,

Here's a question to tickle your brain cells before the weekend

Can I freeze aliquots of Mayer's Haem (home made) for later use? Would
there be any negative effects on composition/staining etc???


--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"There are nights when the wolves are silent and only the moon
howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
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