[Histonet] Perl's iron stain + IF together

[Liz Chlipala]

Kim

I'm not sure about IF but you can do perls with routine IHC with either DAB or AP.  If you are going to use DAB stain the IHC first then stain the perls.  If you are doing AP detection with fast red then stain with perls first and then perform the IHC.  

Liz 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 682-3949
fax (303) 682-9060
liz <@t> premierlab.com
www.premierlab.com
 
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kim Merriam
Sent: Friday, August 15, 2008 10:22 AM
To: Histonet
Subject: [Histonet] Perl's iron stain + IF together

Hello,
Has anyone ever done a Perl's iron stain in conjunction with IF staining for CD markers?  I am not sure which I would need to do first or if one would mask the other.
Any thoughts?
Kim
 Kim Merriam, MA, HT(ASCP)
Cambridge, MA


      
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