[Histonet] Freezing Haematoxylin

[Gayle Callis]

We had Gill hematoxylins sent to us in winter and the stain froze during 
shipment.  This totally ruined the stain, and was an expensive disaster. 
If you look at a Gill's recipe and Mayers, they are similar.  Since then, 
all ordering for hematoxylin in now done in warmer months.   Care is now 
taken to not freeze any hematoxylins.

Gayle M. Callis
HTL/HT/MT(ASCP)
----- Original Message ----- 
From: "Rene J Buesa" <rjbuesa <@t> yahoo.com>
To: <Histonet <@t> lists.utsouthwestern.edu>; "louise renton" 
<louise.renton <@t> gmail.com>
Sent: Friday, August 15, 2008 7:36 AM
Subject: Re: [Histonet] Freezing Haematoxylin


Louise:
You just stepped into "uncharted waters" because except for now when there 
seems to be some hematoxylin shortage, I think that what you ask was never 
tried before.
So, as a present day Magellan, why don't you do the experiment and pass to 
all of us the results?
René J.

--- On Fri, 8/15/08, louise renton <louise.renton <@t> gmail.com> wrote:

From: louise renton <louise.renton <@t> gmail.com>
Subject: [Histonet] Freezing Haematoxylin
To: Histonet <@t> lists.utsouthwestern.edu
Date: Friday, August 15, 2008, 3:41 AM

Dear all,

Here's a question to tickle your brain cells before the weekend

Can I freeze aliquots of Mayer's Haem (home made) for later use? Would
there
be any negative effects on composition/staining etc???


-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"There are nights when the wolves are silent and only the moon
howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
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