[Histonet] Good Cresyl Violet Recipe

[Lee & Peggy Wenk]

Does the post-doc mean cresyl violet acetate, formerly known at cresyl echt
violet? What is the post-doc staining for? Nissl bodies? Pneumocystis?
Nuclei? Mast cell granules? That would determine the exact recipe and
procedure.

Sorry - asking for a recipe for a dye is like asking - does anyone have a
hematoxylin recipe, to which people will say, do you want blue or black
nuclei (Mayer or Gill hematoxylin vs. Weigert)? Do you want to to stain
elastin (VVG)? Do you want to stain myelin (Weil)? All these stain with
hematoxylin, but each one has a different procedure to make up the
hematoxylin stain, and to use it. Same with your cresyl violet.

Sorry, need a little bit more information before we can help.

Peggy A. Wenk HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Chen, Leslie
Sent: Thursday, August 14, 2008 2:48 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Good Cresyl Violet Recipe

Hi Everyone,
My post-doc is asking me for a good cresyl violet recipe.  Does anyone have
one to share?  Thanks!

Leslie Chen

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