detmar <@t> mshri.on.ca
Thu Aug 14 13:46:35 CDT 2008
Hi Michele. I use mouse thymus or spleen controls. I've done caspase-3
IHC on mouse placenta/uterus and you'll get signals, but the problem
with that tissue is that you tend to have different types of cell death
going on (i.e. non-canonical cell death pathways are triggered that
don't use caspase-3), so it can be confusing to interpret the results.
Also, we have found that active caspase-3 is utilized for signaling
pathways other than cell death, so you might see positives that will
likewise, be confusing. Mouse lymphocytes die a nice, normal death
<grin> so using thymus or spleen will give you unambiguous results.
Good luck and if you have any further questions, feel free to ask!
Jacqui Detmar, Post-doctoral Fellow
Samuel Lunenfeld Research Institute,
Mount Sinai Hospital
25 Orde Street, room 6-1001 AJ,
Toronto, ON, Canada
phone: 416-586-4800 x5607
email: detmar <@t> mshri.on.ca
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Michele
Sent: Thursday, August 14, 2008 11:50 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] caspase-3
A couple questions for those of you doing caspase-3 on animal tissue: Is
it necessary to run a negative control tissue? What is a good positive
control tissue? Will mouse ovary/uterus work?
Thanks in advance for any help with this!
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