[Histonet] re-staining an IHC slide for H&E

[Dawson, Glen]

Tiffany,

I would suggest doubling the Hematoxilyn time if the negative went through an enzyme retrieval as this would inhibit the basophillic staining for your H&E.  Otherwise it is actually just as easy as Rene states below.

Good Luck,
Glen Dawson

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Rene J
Buesa
Sent: Wednesday, August 13, 2008 10:27 AM
To: histonet <@t> lists.utsouthwestern.edu; Price, Tiffany
Subject: Re: [Histonet] re-staining an IHC slide for H&E


Just take the coverslip off, hydrate and stain as usual.
René J.

--- On Wed, 8/13/08, Price, Tiffany <Tiffany.Price <@t> thomaswv.org> wrote:

From: Price, Tiffany <Tiffany.Price <@t> thomaswv.org>
Subject: [Histonet] re-staining an IHC slide for H&E
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, August 13, 2008, 9:51 AM

I have been asked to take a negative control slide and re-stain it for
H&E. Does anyone have a protocol for this?
Thanks
Confidentiality Notice:   This email message including any attachments, is for
the sole use of the intended recipient(s) and may contain confidential and
privilege information.   Any unauthorized review, use, disclosure or
distribution is prohibited.   If you are not the intended recipient, please
contact the sender by reply email and destroy ALL copies of the message.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet