[Histonet] uneven ihc staining

Jan Shivers shive003 <@t> umn.edu
Fri Aug 8 08:32:10 CDT 2008

Assuming that you've checked to make sure that the tissues are completely 
covered with reagents during the entire staining process, here's the first 
things that pop into my head as possible answers:

1) tissue partially drying out on the trimming table
2) tissue not completely submerged in formalin after being trimmed in
3) tissue not completely fixed all the way through
4) tissue not completely deparaffinized
5) tissue undergoing faulty antigen retrieval step

Jan Shivers

----- Original Message ----- 
From: "richard shook" <rshooki_99 <@t> yahoo.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, August 08, 2008 7:50 AM
Subject: [Histonet] uneven ihc staining

> Im looking for help we are having uneven staining on some (not all) ihc 
> slides we have looked into several different possoble reasons with no luck 
> ,this happens a couple times every week ao so any suggestion would be 
> great
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