[Histonet] RE: Coverslipper
Rathborne, Toni
trathborne <@t> somerset-healthcare.com
Thu Aug 7 16:54:38 CDT 2008
We have the same. Contact your Leica service dept. We have a great service rep who has a list of what mounting mediums work with which clearants, and what setting the dispenser should be set at. Another thing to watch, is when you replace the bottle with the mounting medium, that may have bubbles in it. To avoid that, we refill ours as soon as we change to another bottle. This way, the newly filled bottle will have a few days to let the bubbles in it rise to the surface. I hope this helps.
Toni Rathborne
Pathology Supervisor
Somerset Medical Center
110 Rehill Ave.
Somerville,NJ 08876
908-595-2367
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
Pemberton, Susan
Sent: Thursday, August 07, 2008 5:30 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Coverslipper
We have a Leica stainer/coverslipper - it's two years old. We like it, but we are having trouble with bubbles and can't seem to adjust them out. Any suggestions?
Susan Pemberton MS,MT(ASCP)SM
Laboratory Administrative Director
Baptist Health
800 Prudential Drive
Jacksonville, FL 32207
904.202.2016
Fax:904.202.2795
susan.pemberton <@t> bmcjax.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Thursday, August 07, 2008 1:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 57, Issue 13
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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. Re: Horse bones (Maxim_71 <@t> mail.ru)
2. RE: protein block (Sally Price)
3. Re: surveillance cameras in the lab (Dawn Cowie)
4. NSH class (Amber McKenzie)
5. Meditech Help (Walter, Janelle)
6. Poster Judges Needed (Cheryl Crowder)
7. RE: HT schools (joelle weaver)
8. Job Opening (Anthony Boris)
9. Coverslipper (Matthew Roark)
10. Re: Coverslipper (Rene J Buesa)
11. GMA 1 micron section staining (Masterson_John)
12. RE: Coverslipper (Bartlett, Jeanine (CDC/CCID/NCZVED))
13. RE: Coverslipper (Mike Pence)
14. RE: Coverslipper (Jackie M O'Connor)
15. Re: GMA 1 micron section staining (Gayle Callis)
16. Job Opening In Ft. Worth Texas (Martinez, Angela)
17. cryostat block holders (Kim Merriam)
18. camera for microscope (Mary Ascenzi)
----------------------------------------------------------------------
Message: 1
Date: Wed, 6 Aug 2008 21:17:28 +0400
From: Maxim_71 <@t> mail.ru
Subject: Re: [Histonet] Horse bones
To: BERGERJL <@t> aol.com
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1446850633.20080806211728 <@t> mail.ru>
Content-Type: text/plain; charset=us-ascii
R. Berger:
I was not be able to get good quality sections from bone
specimens, until begann to use next things:
1- determination endpoint of decalcification
2- isopropanol as dehydratant
3- mineral oil as antemedium before parafiin infiltration.
In my personal experience only these things had great
improvement for quality of my bone sections.
All rest things have had minimal importance for me.
I must use manual processing, because have not any processor.
Sincerely,
Maxim Peshkov,
Russia,
Taganrog.
-----Original message---
> Date: Tue, 5 Aug 2008 20:07:50 EDT
> From: BERGERJL <@t> aol.com
> Subject: [Histonet] Horse bones
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <c8e.277d1ae9.35ca4556 <@t> aol.com>
> Content-Type: text/plain; charset="US-ASCII"
>
> We are trying to section pieces of horse femurs,
> fetlock and carpal joints.
> They have been decalcified with either HCl decal or
> formic acid decal.
> Before routine processing the bones appear to be
> decalcified, but at sectioning
> they are very hard and brittle (even after surface
> decal) and chip out of the
> paraffin block.
> Any suggestions would be helpful. A pathologist
> suggested a soap soaking
> solution but could not remember the name of the soap.
>
> Help and thank you,
>
> R. Berger, HT
------------------------------
Message: 2
Date: Wed, 6 Aug 2008 13:24:45 -0400
From: "Sally Price" <sprice2003 <@t> gmail.com>
Subject: RE: [Histonet] protein block
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<ffd26fa80808061024m59417cc0y87ed2ab0f736919a <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Gene:
What you've decribed is not, in my expericence, that uncommon. It all
depends on the blocking agent your using -- some protein blocks are made
from casein or digested immunoglobulin (e.g Fab2 fargments), some are made
with only animal serum, and others are combinations of these materials -- If
the reagent you're using is made with animal serum, for example, the
backgroud could be caused by the blocking agent sticking to endogenous
immunoglobulins with the specimen. At least this is one possibility, and
the only way to confirm/resolve this issue is to try a differnt type of
protein blocker.
Cheers,
Sally
------------------------------
Message: 16
Date: Tue, 05 Aug 2008 05:49:59 -0400
From: njoydobro <@t> aol.com
Subject: [Histonet] protein block
To: Histonet <@t> lists.utsouthwestern.edu
Good Morning everyone,
???? We are currently working up a new antibody and are trying it with and
without protein block.? We are getting some unexpected results and I would
like to see if anyone has seen similar outcomes.? We are getting less
background without the protein block and the staining appears "crisper".?
Anyone experienced this?
Thanks,
Gene
------------------------------
Message: 3
Date: Wed, 6 Aug 2008 11:29:23 -0700 (PDT)
From: Dawn Cowie <dawn_cowie <@t> yahoo.com>
Subject: Re: [Histonet] surveillance cameras in the lab
To: pruegg <@t> ihctech.net, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, Amos Brooks
<amosbrooks <@t> gmail.com>
Message-ID: <430954.54820.qm <@t> web45013.mail.sp1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I didn't read the initial posts on this topic so I don't know why the lab has installed cameras. I will say that if it is because of problem employees, you get back to the fact of having poor management and supervision in place.
Dawn Cowie, HT
--- On Tue, 8/5/08, Amos Brooks <amosbrooks <@t> gmail.com> wrote:
From: Amos Brooks <amosbrooks <@t> gmail.com>
Subject: [Histonet] surveillance cameras in the lab
To: pruegg <@t> ihctech.net, "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists..utsouthwestern.edu>
Date: Tuesday, August 5, 2008, 9:23 AM
Patsy,
I have to disagree with you there. I understand the need for security,
but there is a certain amount of trust lost when one makes the decision to
monitor employees without even informing them as to why. (Weather or not
they are legally bound to do so.) You can accomplish the same thing by
letting employees know that there is a problem (or a potential problem) and
having them beware and observant. As a last resort when they find the
problem still presists using stromger methods becomes warranted.
Once you give up this personal liberty and allow someone to watch over
your shoulder all the time you loose the ability to make the right choices
as there are no choices to make. I would rather live in a society of people
that want to do the right thing than one of people that are forced to. At
that point we might as well allow those that would do us harm to make all
the decisions for us and abandon any form of personal freedom we may have.
Ben Franklin published in Poor Richard's Almanac the famous quote
*"Those
who would give up essential liberty to purchase a little temporary safety,
deserve neither liberty nor safety."* It is important to temper our fears
with a sense of who we are lest we devolve into a military state.
Thanks,
Amos Brooks
Message: 19
Date: Mon, 4 Aug 2008 10:46:26 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] surveillance cameras in the lab
To: "'Cheri Miller'" <cmiller <@t> physlab.com>,
<Pathrm35 <@t> comcast.net>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<mailman.4.1217869200.32328.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain; charset="us-ascii"
Come on.
In these times of terror concerns I am not sure I would work in a place
where I did not feel secure and the use of these devices help in that
matter.
We built a brand new University of Colorado Health Sciences Center and there
are cameras all over the place as well as lock down. If you do not have an
access card you cannot get into the labs. This was a pain at first but with
all the crazy's we have to worry about out there it now makes me feel
better.
I just read in the paper this morning about a researcher whose house was
bombed by Peta types for doing animal research, and we have had all sorts of
disturbances over the years with precious research animals being released,
protests, etc.
Patsy
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Wed, 6 Aug 2008 14:28:35 -0500
From: "Amber McKenzie" <amber.mckenzie <@t> gastrodocs.net>
Subject: [Histonet] NSH class
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<03C921A1EAF7F541B16543F6EC6A4B3701D3B5C8 <@t> giamail2.Gia.com>
Content-Type: text/plain; charset="US-ASCII"
I am not able to attend the NSH convention this year, and I was looking
at the Program guide...on Sunday from 12:30-4pm is a class "How to Start
an accredited Histotech Program" by Peggy Wenk...anyone know how/if I
could get the information from that class?
Amber McKenzie, B.S., HT (ASCP)
1405 N. State St., Suite 400
Jackson, MS 39202
(ph) 601-863-0388
(fax) 601-326-3532
------------------------------
Message: 5
Date: Wed, 6 Aug 2008 15:30:13 -0400
From: "Walter, Janelle" <walterj <@t> HanoverHospital.org>
Subject: [Histonet] Meditech Help
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E6E8852790CE8C47B38B5793C6C404D9185621 <@t> EXCHANGEHHP1.HHP.PA>
Content-Type: text/plain; charset="US-ASCII"
Is there anyone who I can talk to who has had experience setting up (or
even using) the Meditech PTH module for Histology & Cytology? We are
currently using CoPath, but have been 'sweet-talked' into changing to
Meditech. Any help would be greatly appreciated.
Thanks,
Janelle
Janelle D. Walter, BS MT(ASCP)
Clinical Analyst, MIS
Hanover Hospital
300 Highland Avenue
Hanover, PA 17331
717-646-6899 fax:717-633-3521
walterj <@t> hanoverhospital.org
CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please respond immediately by returning this e-mail to the sender and destroying all copies of this communication including any attachments.
------------------------------
Message: 6
Date: Wed, 06 Aug 2008 16:45:16 -0500
From: "Cheryl Crowder" <ccrowder <@t> vetmed.lsu.edu>
Subject: [Histonet] Poster Judges Needed
To: "Histonet" <histonet <@t> pathology.swmed.edu>
Message-ID: <WorldClient-F200808061645.AA45166308 <@t> vetmed.lsu.edu>
Content-Type: text/plain; charset="us-ascii"
Due to "circumstances beyond our control", 2 of the judged for the NSH
Poster Committee will be unable to attend the Symposium/Convention in
Pittsburgh. So, as chair of the committee, I am looking for 2 volunteers to
judge posters.
Qualifications include NSH membership and attendance at the
Pittsburgh meeting and knowledge of clinical, research and educational
histology. You don't have to have worked in all areas to be familiar with
work done by others. A working knowledge of publication methods is helpful.
The "job" requires that you talk to the presenters Sunday between 10:30
and 12:30, review the posters and judge them in specific areas sometime
between Sunday and Monday afternoon, compile your results (that doesn't take
long) and meet with the other members Monday afternoon.
For all this you do not get paid, stay essentially anonymous and blow
you mind from reviewing outstanding work by our members.
If you are interested, please contact me as soon as possible by e-mail
or phone. As someone said, it's the hardest job you'll ever love.
Cheryl Crowder, BA, HTL(ASCP)
Chief Technologist
Anatomic Pathology
Department of Pathobiological Sciences
School of Veterinary Medicine
Louisiana State University
Skip Bertman Drive
Baton Rouge, LA 70803
225-578-9734
FAX: 225-578-9720
------------------------------
Message: 7
Date: Wed, 6 Aug 2008 22:44:32 +0000
From: joelle weaver <joelleweaver <@t> hotmail.com>
Subject: RE: [Histonet] HT schools
To: <histonet <@t> lists.utsouthwestern.edu>, JD <jdhisto <@t> yahoo.com>
Message-ID: <BAY119-W285CCBAB130896D686B4EED87A0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Those of us who particpate and work in Histology education, do as much as we can! I know that I put in about 20-30 hours a week plus my FT histology job trying to promote histology and being a histotech. For the most part, I get ignored. But just takes MORE people. LOTS more people to particpate.
JMW > From: jdhisto <@t> yahoo.com> Date: Tue, 5 Aug 2008 04:04:13 -0500> To: Histonet <@t> lists.utsouthwestern.edu> CC: > Subject: [Histonet] HT schools> > Hello,> I totally agree with Jennifer Mcdonalds comment. Yes it is all about marketing. People, students , most individuals do not know about Histology. They barely know what the word means, they usually think that its some kind of study of history (go figure). > > I have meet so many students repeatedly (biology, medical technology, chemistry > majors) who love the idea of a career in histotechnology. Especially after finding out the many options there are besides being in a high volume lab cutting 200-300 blocks per day if not more. > > If people dont know...we dont grow. > Till then..> > Sincerely,> JDhisto> _______________________________________________> Histonet mailing list> Histonet <@t> lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_________________________________________________________________
Your PC, mobile phone, and online services work together like never before.
http://clk.atdmt.com/MRT/go/108587394/direct/01/
------------------------------
Message: 8
Date: Thu, 07 Aug 2008 09:18:32 -0400
From: "Anthony Boris" <BORISA <@t> trinity-health.org>
Subject: [Histonet] Job Opening
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <489ABDE8.7813.00DE.0 <@t> trinity-health.org>
Content-Type: text/plain; charset=US-ASCII
St Joseph Mercy Oakland in Pontiac, Michigan has a full time opening for a HT or HTL (ASCP). Registry eligible and new grads are encouraged to apply. It is a day shift position, Monday-Friday. Candidate must be proficient in routine histology skills. Special stain and Immunohistochemistry experience is desired.
The wage scale is highly aggressive and excellent benefits are offered. St Joes even has a free concierge service that will run your errands while you are at work!
Please apply online at http://sjmoweb.trinity-health.org/. or call 248-858-6231 for more info
Thanks
Tony Boris
------------------------------
Message: 9
Date: Thu, 07 Aug 2008 08:27:26 -0500
From: "Matthew Roark" <mroark <@t> sfmc.net>
Subject: [Histonet] Coverslipper
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <489AB1EE02000011000665B6 <@t> email_gateway.sfmc.net>
Content-Type: text/plain; charset=US-ASCII
We are currently in the market for a new coverslipper. What are you
using and/or what would everyone recommend?
We currently have an old Leica glass coverslipper but would be open to
try something new.
Thanks!
Matthew Roark
Histology Specialist -B.S,HT(ASCP)CM
Saint Francis Medical Center
211 Saint Francis Drive
Cape Girardeau, MO 63703
573-331-5267
------------------------------
Message: 10
Date: Thu, 7 Aug 2008 06:44:18 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Coverslipper
To: Histonet <@t> lists.utsouthwestern.edu, Matthew Roark <mroark <@t> sfmc.net>
Message-ID: <274640.66247.qm <@t> web65710.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Try the Sakura film, or if you prefer glass, the Sakura glass coverslipper.
René J.
--- On Thu, 8/7/08, Matthew Roark <mroark <@t> sfmc.net> wrote:
From: Matthew Roark <mroark <@t> sfmc.net>
Subject: [Histonet] Coverslipper
To: Histonet <@t> lists.utsouthwestern.edu
Date: Thursday, August 7, 2008, 9:27 AM
We are currently in the market for a new coverslipper. What are you
using and/or what would everyone recommend?
We currently have an old Leica glass coverslipper but would be open to
try something new.
Thanks!
Matthew Roark
Histology Specialist -B.S,HT(ASCP)CM
Saint Francis Medical Center
211 Saint Francis Drive
Cape Girardeau, MO 63703
573-331-5267
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Thu, 7 Aug 2008 06:33:43 -0700
From: "Masterson_John" <Masterson_John <@t> Allergan.com>
Subject: [Histonet] GMA 1 micron section staining
To: "Anthony Boris" <BORISA <@t> trinity-health.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<0C58C4F16F0B67448318A38041CADE4B01594F26 <@t> IRMAIL133.irvine.allergan.com>
Content-Type: text/plain; charset="us-ascii"
Hello Histonetters,
I need to stain some 1 micron sections of GMA embedded tissue for H&E.
Does anyone have experience they can share? Thanks in advance.
John
------------------------------
Message: 12
Date: Thu, 7 Aug 2008 09:48:27 -0400
From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" <jqb7 <@t> cdc.gov>
Subject: RE: [Histonet] Coverslipper
To: "Matthew Roark" <mroark <@t> sfmc.net>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<1CE1847DFEA0A647B1CCDE4108EA60A70208BF30 <@t> LTA3VS011.ees.hhs.gov>
Content-Type: text/plain; charset=us-ascii
We have one of the newer Leica's and have just recently ordered one of
Sakura's as well. We have not yet tried the Sakura brand. We have to
have glass and have been very pleased with the Leica.
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Matthew
Roark
Sent: Thursday, August 07, 2008 9:27 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Coverslipper
We are currently in the market for a new coverslipper. What are you
using and/or what would everyone recommend?
We currently have an old Leica glass coverslipper but would be open to
try something new.
Thanks!
Matthew Roark
Histology Specialist -B.S,HT(ASCP)CM
Saint Francis Medical Center
211 Saint Francis Drive
Cape Girardeau, MO 63703
573-331-5267
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 13
Date: Thu, 7 Aug 2008 09:01:59 -0500
From: "Mike Pence" <mpence <@t> grhs.net>
Subject: RE: [Histonet] Coverslipper
To: "Matthew Roark" <mroark <@t> sfmc.net>,
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<661949901A768E4F9CC16D8AF8F2838C017A38CB <@t> IS-E2K3.grhs.net>
Content-Type: text/plain; charset="us-ascii"
I love our Sakura film. Have had no problems with it in over 10 years,
just general maintenance.
Mike
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Matthew
Roark
Sent: Thursday, August 07, 2008 8:27 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Coverslipper
We are currently in the market for a new coverslipper. What are you
using and/or what would everyone recommend?
We currently have an old Leica glass coverslipper but would be open to
try something new.
Thanks!
Matthew Roark
Histology Specialist -B.S,HT(ASCP)CM
Saint Francis Medical Center
211 Saint Francis Drive
Cape Girardeau, MO 63703
573-331-5267
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Thu, 7 Aug 2008 09:12:07 -0500
From: Jackie M O'Connor <Jackie.O'Connor <@t> abbott.com>
Subject: RE: [Histonet] Coverslipper
To: "Mike Pence" <mpence <@t> grhs.net>
Cc: Histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OF358D7747.CA68A802-ON8625749E.004DE2D4-8625749E.004E090D <@t> abbott.com>
Content-Type: text/plain; charset="US-ASCII"
We have been using the Sakura film coverslipper for over a year - slides
can be put into slide racks within minutes after coverslipping. Great
when you have to turn slides around or mail them out quickly.
Jackie O'
"Mike Pence" <mpence <@t> grhs.net>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
08/07/2008 09:01 AM
To
"Matthew Roark" <mroark <@t> sfmc.net>, <Histonet <@t> lists.utsouthwestern.edu>
cc
Subject
RE: [Histonet] Coverslipper
I love our Sakura film. Have had no problems with it in over 10 years,
just general maintenance.
Mike
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Matthew
Roark
Sent: Thursday, August 07, 2008 8:27 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Coverslipper
We are currently in the market for a new coverslipper. What are you
using and/or what would everyone recommend?
We currently have an old Leica glass coverslipper but would be open to
try something new.
Thanks!
Matthew Roark
Histology Specialist -B.S,HT(ASCP)CM
Saint Francis Medical Center
211 Saint Francis Drive
Cape Girardeau, MO 63703
573-331-5267
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Thu, 7 Aug 2008 09:12:31 -0600
From: "Gayle Callis" <gayle.callis <@t> bresnan.net>
Subject: Re: [Histonet] GMA 1 micron section staining
To: "Masterson_John" <Masterson_John <@t> Allergan.com>, "Anthony Boris"
<BORISA <@t> trinity-health.org>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001d01c8f8a0$035fb980$6501a8c0 <@t> Sunney>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Do not rinse but put dry sections directly to Gill 3 hematoxylin 10 minutes.
Time can vary.
Rinse 3 X with distilled water
Go directly to bluing 1 min Scotts Tap water substitute
Rinse with distilled water
Air dry sections
Eosin/phloxine for 2 to 5 minutes time will vary.
Rinse very quickly through 2 changes of 95% ethanol only and air dry
quickly. I liked to use compressed or forced air, a fan works nicely.
Coverslip with permanent mounting media over dry section.
Be aware that 1 um is really thin and will not look like a regular H&E on a
thicker section. There just isn't much tissue in a 1 um thick section. I
had pathologists complain, but when this was explained, they understood what
was going on since they were used to looking at 5 um thick sections.
Alcohols during dehyration will cause sections to release or bulge from
slide. Xylene can create problems too so we always mounted a coverglass
over a dry section. You can thin the mounting media a bit just in case it
doesn't flow well. If you have a bit of funky plastic fold over of plastic,
that can be scraped away with a teflon coated razor blade, to not have extra
thickness in a bad place during coverslipping.
We did not use Harris hematoxylin for GMA sections, and this method
basically is what Polysciences put out years ago when GMA first came out,
and it worked very well for us over the years.
Good luck on staining
Gayle M. Callis
HTL/HT/MT(ASCP)
----- Original Message -----
From: "Masterson_John" <Masterson_John <@t> Allergan.com>
To: "Anthony Boris" <BORISA <@t> trinity-health.org>;
<histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, August 07, 2008 7:33 AM
Subject: [Histonet] GMA 1 micron section staining
Hello Histonetters,
I need to stain some 1 micron sections of GMA embedded tissue for H&E.
Does anyone have experience they can share? Thanks in advance.
John
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------------------------------
Message: 16
Date: Thu, 7 Aug 2008 11:20:01 -0500
From: "Martinez, Angela" <amartinez <@t> carisdx.com>
Subject: [Histonet] Job Opening In Ft. Worth Texas
To: <histonet <@t> lists.utsouthwestern.edu>
Cc: "Hale, Meredith" <mhale <@t> carisdx.com>
Message-ID:
<9B8A3AC772C7F64680392A7CB8FBFB0F05549E1D <@t> s-irv-ex301.PathologyPartners.intranet>
Content-Type: text/plain; charset="us-ascii"
Great opportunity for Histotechnician in brand new laboratory!
Gastrointestinal Associates of North Texas in Ft Worth , is looking for
a certified HT or HTL to run their newly constructed laboratory.
Candidate must be ASCP certified and meet CLIA-88 regulations to perform
gross dissection. Prior supervisory experience preferred. The candidate
will be responsible for the following: Creation and maintenance of
policies and procedures to CLIA standards, leading lab through CLIA
inspection, maintenance and quality control for equipment, and routine
histology duties. The position offers competitive salary, medical
insurance, retirement plan, and vacation /sick leave. Interested
applicants should e-mail resumes to Meredith Hale at mhale <@t> carisdx.com .
Meredith Hale HT (ASCP)
Technical Director
Caris Diagnostics
8400 Esters Blvd. Ste. 190
Irving, Texas 75063
------------------------------
Message: 17
Date: Thu, 7 Aug 2008 09:36:02 -0700 (PDT)
From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
Subject: [Histonet] cryostat block holders
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <611208.37592.qm <@t> web50305.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi everyone,
I want to order some disposable cryostat block holders (the round things that you attach your block to). I don't know what they are officially called, so I am having trouble searching for them. Does anyone know what they are called and if anyone sells plastic ones?
Thanks,
Kim
Kim Merriam, MA, HT(ASCP)
Cambridge, MA
------------------------------
Message: 18
Date: Thu, 07 Aug 2008 12:43:14 -0400
From: Mary Ascenzi <maa8 <@t> cornell.edu>
Subject: [Histonet] camera for microscope
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.2.1.2.2.20080807123426.01e0a900 <@t> postoffice6.mail.cornell.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Hello All
I have an old Olympus microscope and am looking for a digital camera for
photomics of slides.
I know that there are cameras designed specifically for microscopes, but am
wondering if
anyone has used a digital SLR with an adapter.
What is the photo quality, ie sharpness, color fidelity ?
What software do you use ? Is Photoshop adequate?
thanks,
Mary
------------------------------
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End of Histonet Digest, Vol 57, Issue 13
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