[Histonet] Microglia antibody

[Gayle Callis]

It is specific antibody for mouse and not rat.  People have used it with 
FFPE tissue.  This has been put on Histonet several times, including the 
retrieval you need to use.  We only use it with cryostat sections, our 
special acetone alcohol fixation.  For fluoescence work, we only use 
cryostat sections with this fixative to avoid aldehyde induced 
autofluorescence.  If one uses it for IFA, then it would be wise to remove 
free aldehydes to reduce the autofluorescence problem.

Gayle M. Callis
HTL/HT/MT(ASCP)


----- Original Message ----- 
From: "Caroline Bass" <cbass <@t> wfubmc.edu>
To: "Geoff McAuliffe" <mcauliff <@t> umdnj.edu>
Cc: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, August 07, 2008 11:31 AM
Subject: Re: [Histonet] Microglia antibody


Thanks for the suggestions.  I've used F4/80 in the past, I think this one
was from serotec, but I used it in mouse sections.

Does anyone have experience with this antigen or antibody?

Thanks!


On 8/7/08 1:29 PM, "Geoff McAuliffe" <mcauliff <@t> umdnj.edu> wrote:

> Hi Caroline:
>
>     Both ED-1 and OX42 stain rat brain microglia, but different
> populations. I will try to dig up a reference for you.
>
> Geoff
>
> Caroline Bass wrote:
>> Hello Everyone,
>>
>> I¹m looking for an antibody to stain microglia in rat brain sections. 
>> These
>> will be perfused with formalin and sectioned at about 60 microns.  I¹ll
>> probably start with an ABC kit, but would also like the ability to use 
>> the
>> antibody for immunofluorescence. Are there any recommendations?
>>
>> Thanks,
>>
>> Caroline Bass
>>
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>>
>>
>


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