[Histonet] Microglia antibody
Nancy Lemke
nsnwl <@t> neuro.hfh.edu
Thu Aug 7 14:10:54 CDT 2008
Hi Caroline,
I use Serotec CD68 (MCA341R) with wonderful results. I retrieve in citrate buffer pH 6.0 on a hotplate (boil 10 and cool 20), and dilute the antibody to 1:200, incubate for 30 minutes and use Biocare 4+ avidin-biotinHRP detection and DAB. The stain is very clean and reliable.
Nancy Lemke
Nancy Lemke
Research Coordinator
Hermelin Brain Tumor Center
Henry Ford Hospital
Detroit
-----Original message-----
From: "Liz Chlipala" liz <@t> premierlab.com
Date: Thu, 07 Aug 2008 13:25:09 -0400
To: "Caroline Bass" cbass <@t> wfubmc.edu
Subject: RE: [Histonet] Microglia antibody
> Caroline
>
> I believe you can use ED-1 or OX42 or both. Both work in FFPE tissue > samples.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Manager
> Premier Laboratory, LLC
> P.O. Box 18592
> Boulder, CO 80308
> phone (303) 682-3949
> fax (303) 682-9060
> liz <@t> premierlab.com
> www.premierlab.com
>
> Ship to Address:
>
> Premier Laboratory, LLC
> 1567 Skyway Drive
> Unit E
> Longmont, CO 80504
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu > [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Caroline > Bass
> Sent: Thursday, August 07, 2008 11:13 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Microglia antibody
>
> Hello Everyone,
>
> I¹m looking for an antibody to stain microglia in rat brain sections. > These will be perfused with formalin and sectioned at about 60 microns. > I¹ll probably start with an ABC kit, but would also like the ability > to use the antibody for immunofluorescence. Are there any > recommendations?
>
> Thanks,
>
> Caroline Bass
>
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