[Histonet] Horse bones

Jackie M O'Connor Jackie.O'Connor <@t> abbott.com
Wed Aug 6 08:07:55 CDT 2008

Consider your processing schedule as well.   Is it long enough to 
infiltrate large bones?

"Piero Nelva" <pieronelva01 <@t> bigpond.com> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
08/06/2008 03:42 AM

<Histonet <@t> lists.utsouthwestern.edu>

Re: [Histonet] Horse bones

For tough or brittle, but not necessarily calcified tissue, I find that 
soaking the block on a water soaked tissue for 10-15 minutes effective for 

softening.  It can sometimes take all day to get a section, as trimming 
sometimes has to be done 4-5 microns at a time!!

Piero Nelva
Monash Medical Centre
----- Original Message ----- 
From: "louise renton" <louise.renton <@t> gmail.com>
To: <BERGERJL <@t> aol.com>; <Histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, August 06, 2008 5:58 PM
Subject: Re: [Histonet] Horse bones

> Some things to think about....
> Rinse the tissue well with running tap water after decal and before
> processing - this seems to make  a difference (depends on the size of 
> bone - 2hrs to overnight)
> EMbed the bones so that the long axis is either at right angles or 45
> degrees to the blade so that the edge of the knife doesn't have to cut
> through a narrow band of cortical bone along its entire length - this 
> causes
> "skipping"(If this is unclear I can send you an attachment with a 
> diagramme)
> Once you have faced the blocks put the face down on ice in a -20 deg 
> freezer
> overnight (chest type freezer). then remove the one ata time for 
> sectioning.
> I have only had limited sucess with soaking the  faced blocks in fabric
> softener undiluted.
> Hopes this helps
> best regards
> On 8/6/08, BERGERJL <@t> aol.com <BERGERJL <@t> aol.com> wrote:
>> We are trying to section pieces of horse femurs, fetlock and
>> carpal  joints.
>> They have been decalcified with either HCl decal or formic acid  decal.
>> Before routine processing the bones appear to be decalcified, but  at
>> sectioning
>> they are very hard and brittle (even after surface decal) and chip  out 

>> of
>> the
>> paraffin block.
>> Any suggestions would be helpful.  A pathologist suggested a soap 
>> soaking
>> solution but could not remember the name of the soap.
>> Help and thank you,
>> R. Berger, HT
>> **************Looking for a car that's sporty, fun and fits in your 
>> budget?
>> Read reviews on AOL Autos.
>> (
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> -- 
> Louise Renton
> Bone Research Unit
> University of the Witwatersrand
> Johannesburg
> South Africa
> "There are nights when the wolves are silent and only the moon howls".
> George Carlin
> No trees were killed in the sending of this message.
> However, many electrons were terribly inconvenienced.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> No virus found in this incoming message.
> Checked by AVG - http://www.avg.com
> Version: 8.0.138 / Virus Database: 270.5.12/1594 - Release Date: 
> 9:49 PM

Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu

More information about the Histonet mailing list