[Histonet] p53 staining pattern

[Aprill Watanabe]

I have an intellectual question for you all.  I have recently stained a
colon tumor with p53 from Novocastra and got clear areas of positive and
negative staining for no visible reason (i.e. Normal tissue vs. tumor
tissue).  It looks like a fixation issue but in some areas there will be no
staining then a small group of cells (like an island) will stain.  I¹m not
sure how to check if this is a biological function or if this is caused by
something pre-analytical.  Can anyone help?

Aprill Watanabe, B.S.
Research Associate
Integrated Cancer Genomics Division
Tissue Microarray Center (TMA)
Translational Genomics Research Institute (TGen)
main: 602-343-8822
Fax: 602-343-8840
awatanabe <@t> tgen.org
www.tgen.org