[Histonet] RE: Histonet Digest, Vol 57, Issue 5, re:p16 on ventana

Mon Aug 4 13:56:07 CDT 2008

Hello All, 

We used the following protocol at PH for p16 you can use the check marks in
the protocol to signify selection: 
Paraffin- (selected) 
Deparaffinization- (selected) 
Cell Conditioning - (selected)
Cell Conditioner #1- (selected)
Mild CC1- (selected)  
Antibody- (selected)
Apply 1 drop of [prep kit # designation for p16](Antibody) and incubate for
{0 hr 32 min)
The rest is up to you to include counter stain or not(usually hematoxylin)
Ours was predilute from Cell Marque and we used a prep kit number.  

Good luck and have a great day. 
Valantou Grover, HT(ASCP), HTL, PA
Biosciences Product Line Manager 
Polysciences, Inc.  
-----Original Message-----
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Subject: Histonet Digest, Vol 57, Issue 5

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Today's Topics:

   1. surveillance cameras in the lab (Paula Pierce)
   2. Re: surveillance cameras in the lab (Bill)
   3. surveillance cameras in the lab (Paula Pierce)
   4. RE: surveillance cameras in the lab
      (Bartlett, Jeanine (CDC/CCID/NCZVED))
   5. RE: surveillance cameras in the lab (Weber, Susan (VHACLE))
   6. RE: surveillance cameras in the lab (Martin, Gary)
   7. RE: tissue falling out of paraffin block (Monfils, Paul)
   8. PTAH staining on Carnoy's fixed tissue (Trajkovic, Dusko)
   9. p16 on Ventana (Sharon.Davis-Devine)
  10. surveillance cameras in the lab (Troutman, Kenneth A)
  11. stainless steel bucket (Amber McKenzie)
  12. RE: p16 on Ventana (Patsy Ruegg)
  13. Tissue Microarray Location Markers (Thom Jensen)
  14. RE: surveillance cameras in the lab (Patsy Ruegg)
  15. RE: tissue falling out of paraffin block (Patsy Ruegg)
  16. RE: stainless steel bucket (Bonner, Janet)
  17. Re: Rabbit IgG control (R C)
  18. RE: HT schools (Patsy Ruegg)
  19. RE: surveillance cameras in the lab (Patsy Ruegg)
  20. QIHC Certification (William Connor)

----------------------------------------------------------------------

Message: 1
Date: Mon, 4 Aug 2008 07:02:12 -0700 (PDT)
From: Paula Pierce <contact <@t> excaliburpathology.com>
Subject: [Histonet] surveillance cameras in the lab
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <419055.10912.qm <@t> web50106.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

I thought about blacking out the lenses too.
The worst part of this whole situation is that the cameras suddenly
appeared. The tech just happened to notice them. It is totally unacceptable
to me that an institution would not inform the employees they were going to
install cameras. This may be CYA for the powers that be. 
RUN!

 Paula Pierce, HTL(ASCP)HT

Excalibur Pathology, Inc. 
631 N. Broadway Ave.
Moore, OK 73160 
405-570-6679 cell
405-759-3953 lab
contact <@t> excaliburpathology.com
www.excaliburpathology.com

------------------------------

Message: 2
Date: Mon, 4 Aug 2008 09:02:27 -0500
From: Bill <bill501 <@t> mindspring.com>
Subject: Re: [Histonet] surveillance cameras in the lab
To: Pathrm35 <@t> comcast.net, histonet <@t> lists.utsouthwestern.edu
Message-ID: <p06240804c4bcbc06aeef@[4.244.150.186]>
Content-Type: text/plain; charset="us-ascii"

At 10:21 AM +0000 8/4/08, Pathrm35 <@t> comcast.net wrote:
>>>I was wondering how many techs out there have cameras in their labs,
either for security or to monitor employees. I went to work Sunday night and
noticed that 4 cameras were installed in the lab over the weekend, with more
to come.<<<

I would use immersion oil on the camera lenses. 

-- 
______________
Bill Blank, MD
Heartland Lab

------------------------------

Message: 3
Date: Mon, 4 Aug 2008 07:03:53 -0700 (PDT)
From: Paula Pierce <contact <@t> excaliburpathology.com>
Subject: [Histonet] surveillance cameras in the lab
To: Rosa Fields <rfields <@t> gidocs.net>,	Histonet
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <499718.21489.qm <@t> web50109.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hydrofluoric acid etches glass ;)

 Paula Pierce, HTL(ASCP)HT

Excalibur Pathology, Inc. 
631 N. Broadway Ave.
Moore, OK 73160 
405-570-6679 cell
405-759-3953 lab
contact <@t> excaliburpathology.com
www.excaliburpathology.com

----- Original Message ----
From: Rosa Fields <rfields <@t> gidocs.net>
To: Mike Pence <mpence <@t> grhs.net>; Pathrm35 <@t> comcast.net;
histonet <@t> lists.utsouthwestern.edu
Sent: Monday, August 4, 2008 8:46:02 AM
Subject: RE: [Histonet] surveillance cameras in the lab

You could put a nice coat of paraffin on the lenses!

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mike
Pence
Sent: Monday, August 04, 2008 8:31 AM
To: Pathrm35 <@t> comcast.net; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] surveillance cameras in the lab

One way of finding out who is "watching you" is to blackout the lenses.
This should get a quick response!

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Pathrm35 <@t> comcast.net
Sent: Monday, August 04, 2008 5:21 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] surveillance cameras in the lab

I was wondering how many techs out there have cameras in their labs,
either for security or to monitor employees. I went to work Sunday night
and noticed that 4 cameras were installed in the lab over the weekend,
with more to come. _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
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------------------------------

Message: 4
Date: Mon, 4 Aug 2008 10:17:38 -0400
From: "Bartlett, Jeanine (CDC/CCID/NCZVED)" <jqb7 <@t> cdc.gov>
Subject: RE: [Histonet] surveillance cameras in the lab
To: "Paula Pierce" <contact <@t> excaliburpathology.com>,	Histonet
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<1CE1847DFEA0A647B1CCDE4108EA60A7F23E22 <@t> LTA3VS011.ees.hhs.gov>
Content-Type: text/plain; charset=iso-8859-1

I am not against the idea of the cameras...but I would definitely have an
issue with no one being notified that they were going to be installed and
why. 

Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartlett <@t> cdc.hhs.gov

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Monday, August 04, 2008 10:02 AM
To: Histonet
Subject: [Histonet] surveillance cameras in the lab

I thought about blacking out the lenses too.
The worst part of this whole situation is that the cameras suddenly
appeared. The tech just happened to notice them. It is totally unacceptable
to me that an institution would not inform the employees they were going to
install cameras. This may be CYA for the powers that be. 
RUN!

 Paula Pierce, HTL(ASCP)HT

Excalibur Pathology, Inc. 
631 N. Broadway Ave.
Moore, OK 73160
405-570-6679 cell
405-759-3953 lab
contact <@t> excaliburpathology.com
www.excaliburpathology.com
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 5
Date: Mon, 4 Aug 2008 10:34:05 -0400
From: "Weber, Susan (VHACLE)" <Susan.Weber2 <@t> va.gov>
Subject: RE: [Histonet] surveillance cameras in the lab
To: "Bartlett, Jeanine (CDC/CCID/NCZVED)" <jqb7 <@t> cdc.gov>,	"Paula
	Pierce" <contact <@t> excaliburpathology.com>,	"Histonet"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:

<16C83872A53F4346AA9C3A18E3A3AAB903F76E15 <@t> VHAV10MSGA1.v10.med.va.gov>
Content-Type: text/plain;	charset="iso-8859-1"

Was this through out the whole lab, or just Histology. The other departments
may be interested in knowing they are being "observed" dare I say "watched",
"spied upon", etc. It is highly unacceptable to me that there is no
notification to the "workers" that big bro is hovering overhead. Also
unacceptable is the fact that "they" are not giving a very good "reason" for
the install. BTW- wear proper PPE so they can't tell who is "cleaning" that
lens with the nice immersion oil. 

Susan M Weber HT(ASCP)
Histology Supervisor
Louis Stokes Cleveland VA Medical Center
10701 East Blvd
Cleveland, Ohio 44106
(216) 791-3800 X6154
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bartlett,
Jeanine (CDC/CCID/NCZVED)
Sent: Monday, August 04, 2008 10:18 AM
To: Paula Pierce; Histonet
Subject: RE: [Histonet] surveillance cameras in the lab

I am not against the idea of the cameras...but I would definitely have an
issue with no one being notified that they were going to be installed and
why. 

Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590 
jeanine.bartlett <@t> cdc.hhs.gov

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Monday, August 04, 2008 10:02 AM
To: Histonet
Subject: [Histonet] surveillance cameras in the lab

I thought about blacking out the lenses too.
The worst part of this whole situation is that the cameras suddenly
appeared. The tech just happened to notice them. It is totally unacceptable
to me that an institution would not inform the employees they were going to
install cameras. This may be CYA for the powers that be. 
RUN!

 Paula Pierce, HTL(ASCP)HT

Excalibur Pathology, Inc. 
631 N. Broadway Ave.
Moore, OK 73160
405-570-6679 cell
405-759-3953 lab
contact <@t> excaliburpathology.com
www.excaliburpathology.com
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 6
Date: Mon, 4 Aug 2008 07:42:47 -0700
From: "Martin, Gary" <gmartin <@t> marshallmedical.org>
Subject: RE: [Histonet] surveillance cameras in the lab
To: "Paula Pierce" <contact <@t> excaliburpathology.com>,	"Histonet"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<6ED9D4252F278841A0593D3D788AF24C02FC5A1F <@t> mailsvr.MARSHMED.local>
Content-Type: text/plain;	charset="iso-8859-1"

The camera thing is odd! There should be some sort of written policy to
accompany this process.  I was a school board member for several years and
the other members insisted on installing cameras in the buss'.  This, as one
responder mentioned, caused all kinds of unanticipated problems. The first
and foremost was that all information gathered was after the fact and the
images were not clear enough to assign blame.  Also it was surprising who
got their hand on those recordings for their viewing pleasure.  Needless to
say the very expensive cameras were removed after two lawsuits by parents.
I do agree with one of the responders ... that this is something the
administration is not handling directly.  They at least owe you an
explanation! 
Gary

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Monday, August 04, 2008 7:02 AM
To: Histonet
Subject: [Histonet] surveillance cameras in the lab

I thought about blacking out the lenses too.
The worst part of this whole situation is that the cameras suddenly
appeared. The tech just happened to notice them. It is totally unacceptable
to me that an institution would not inform the employees they were going to
install cameras. This may be CYA for the powers that be. 
RUN!

 Paula Pierce, HTL(ASCP)HT

Excalibur Pathology, Inc. 
631 N. Broadway Ave.
Moore, OK 73160 
405-570-6679 cell
405-759-3953 lab
contact <@t> excaliburpathology.com
www.excaliburpathology.com
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 7
Date: Mon, 4 Aug 2008 10:45:40 -0400
From: "Monfils, Paul" <PMonfils <@t> Lifespan.org>
Subject: RE: [Histonet] tissue falling out of paraffin block
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:

<4EBFF65383B74D49995298C4976D1D5E03835C45 <@t> LSRIEXCH1.lsmaster.lifespan.org>

Content-Type: text/plain;	charset="iso-8859-1"

That sounds like incomplete dehydration.  A small amount of water remains in
the tissue.  This prevents full infiltration by both the clearling agent and
the paraffin.  Once the block is faced off, the water and/or solvent
evaporates from the tissue, causing it to shrink and pull away from the
paraffin.

> ----------
> From: 	histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
Price, Tiffany
> Sent: 	Monday, August 4, 2008 9:56 AM
> To: 	histonet <@t> lists.utsouthwestern.edu
> Subject: 	[Histonet] tissue falling out of paraffin block
> 
> Can anyone tell me what would make liver tissue fall out of a paraffin
> block after routine processing? We cut a routine H&E slide, and later,
> had to cut IHC stains that were ordered. The tissue was fine for the
> H&E, but the whole tissue fell out of the block when we tried to re-cut
> it, even after re-embedding. 
>  
> Thanks for any help
> Tiffany
> Confidentiality Notice:   This email message including any attachments, is
for the sole use of the intended recipient(s) and may contain confidential
and privilege information.   Any unauthorized review, use, disclosure or
distribution is prohibited.   If you are not the intended recipient, please
contact the sender by reply email and destroy ALL copies of the message.
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 

------------------------------

Message: 8
Date: Mon, 4 Aug 2008 07:49:29 -0700
From: "Trajkovic, Dusko" <dusko.trajkovic <@t> pfizer.com>
Subject: [Histonet] PTAH staining on Carnoy's fixed tissue
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:

<3AD0BD3142459B4E9B12CBEAFF2B89B207AC423B <@t> lajamrexm01.amer.pfizer.com>
Content-Type: text/plain;	charset="us-ascii"

Good Morning/Day/Evening Histonet colleagues,
Does anyone have a protocol for staining PTAH on Carnoy's fixed tissue?
How does the fixative impact the PTAH staining? 
Any information would be greatly appreciated.
Thank you,
Dusko Trajkovic

------------------------------

Message: 9
Date: Mon, 4 Aug 2008 09:59:33 -0500
From: "Sharon.Davis-Devine" <Sharon.Davis-Devine <@t> carle.com>
Subject: [Histonet] p16 on Ventana
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<44780C571F28624DBB446DE55C4D733A021E0A51 <@t> EXCHANGEBE1.carle.com>
Content-Type: text/plain;	charset="us-ascii"

Hey Histonetters!  Is anyone out there in histoworld running p16 on the
Ventana system?  If so, can you provide us with your protocol and where
you are ordering it from?  We are aware that MTM is presently the only
vendor for p16 but it is rather pricey and are wondering if there are
any alternatives available out there.  Once again thank you so much for
your help.  

Sharon Davis-Devine, CT (ASCP)

Cytology Supervisor

Carle Clinic

602 West University

Urbana, Illinois 61801

Phone:  217-383-3572

Email:  sharon.davis-devine <@t> carle.com

------------------------------

Message: 10
Date: Mon, 4 Aug 2008 11:03:17 -0500
From: "Troutman, Kenneth A" <kenneth.a.troutman <@t> Vanderbilt.Edu>
Subject: [Histonet] surveillance cameras in the lab
To: "Histonet" <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:

<37DEF9AF72994947AF693956A59B9B660127FF52 <@t> mailbe03.mc.vanderbilt.edu>
Content-Type: text/plain;	charset="iso-8859-1"

I used to work in a lab that had surveillance cameras.  At the time, we only
had one that was over our single grossing station to monitor grossing.
(There were some missing tissue accusations that needed to be nipped in the
bud, so to say...)  I eventually started managing the accessioning
department and we installed 4 to monitor specimen receipt to see if anything
was accidently thrown in the trash.  It was supposed to be a quick reference
tool that was designed to keep us from digging in the biohazard trash
unnecessarily, however, it proved to be more useful for catching employees
napping!  (Yes, they were all aware that the cameras existed!)  

As for cameras in the lab in general, you will probably have to ask upper
management (or whoever made the decision to install them) why they are
there.  Perhaps they may even tell you the answer.

Good luck.

Ashley Troutman BS, HT(ASCP)QIHC
Histopathology Laboratory
Department of Pathology
Vanderbilt University Medical Center
Nashville, TN
<http://www.vanderbilthealth.com/main/> <http://www.vanderbilt.edu/>
<http://www.mc.vanderbilt.edu/> <http://www.vanderbilthealth.com/main/>  

------------------------------

Message: 11
Date: Mon, 4 Aug 2008 11:06:26 -0500
From: "Amber McKenzie" <amber.mckenzie <@t> gastrodocs.net>
Subject: [Histonet] stainless steel bucket
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<03C921A1EAF7F541B16543F6EC6A4B3701D3B09F <@t> giamail2.Gia.com>
Content-Type: text/plain;	charset="US-ASCII"

Does anyone know where I can purchase a stainless steel basket/bucket to
wash my cassette lids in?  We use xylene and Liqui-Nox to wash them each
day and our plastic buckets eventually end up tearing and breaking over
time and I have to constantly replace them, so I'm looking for something
more stable like stainless steel. 

------------------------------

Message: 12
Date: Mon, 4 Aug 2008 10:07:15 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] p16 on Ventana
To: "'Sharon.Davis-Devine'" <Sharon.Davis-Devine <@t> carle.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<mailman.0.1217869200.32328.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain;	charset="us-ascii"

Sharon,
The NSH IHC Resource Group just had a discussion about p16 and some
alternatives were offered.  NSH members can join the ihcrg online at
www.ihcrg.org 
Best regards,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
pruegg <@t> ihctech.net
www.ihctech.net
www.ihcrg.org    

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Sharon.Davis-Devine
Sent: Monday, August 04, 2008 9:00 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] p16 on Ventana

Hey Histonetters!  Is anyone out there in histoworld running p16 on the
Ventana system?  If so, can you provide us with your protocol and where
you are ordering it from?  We are aware that MTM is presently the only
vendor for p16 but it is rather pricey and are wondering if there are
any alternatives available out there.  Once again thank you so much for
your help.  

Sharon Davis-Devine, CT (ASCP)

Cytology Supervisor

Carle Clinic

602 West University

Urbana, Illinois 61801

Phone:  217-383-3572

Email:  sharon.davis-devine <@t> carle.com

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 13
Date: Mon, 4 Aug 2008 16:12:27 +0000
From: Thom Jensen <tissuearray <@t> hotmail.com>
Subject: [Histonet] Tissue Microarray Location Markers
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BAY115-W5233D41BC0ACDD15276615BB780 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"

I am trying to find out what the best way is to determine the number one
spot on a TMA?  We use died lung for location markers.  One color punch
represents a group of punches.  What other ways are techs marking their TMA
locations?  I know there are a variety of ways for documentation of TMAs but
I am interested in the TMA block and location marker techniques and
materials.

Thanks all,
Thom   HT (ASAP) TMA Technician

New TMA instrument go to: arraymold.com
_________________________________________________________________
Your PC, mobile phone, and online services work together like never before.
http://clk.atdmt.com/MRT/go/108587394/direct/01/

------------------------------

Message: 14
Date: Mon, 4 Aug 2008 10:15:57 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] surveillance cameras in the lab
To: "'Bill'" <bill501 <@t> mindspring.com>, <Pathrm35 <@t> comcast.net>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<mailman.1.1217869200.32328.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain;	charset="us-ascii"

As an employer I am unaware of any regulations requiring you to inform
employees that you will install cameras for security or any other reason,
you would probably need to post a sign stating that there are cameras being
used in the area is all?
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
pruegg <@t> ihctech.net
www.ihctech.net
www.ihcrg.org    

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bill
Sent: Monday, August 04, 2008 8:02 AM
To: Pathrm35 <@t> comcast.net; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] surveillance cameras in the lab

At 10:21 AM +0000 8/4/08, Pathrm35 <@t> comcast.net wrote:
>>>I was wondering how many techs out there have cameras in their labs,
either for security or to monitor employees. I went to work Sunday night and
noticed that 4 cameras were installed in the lab over the weekend, with more
to come.<<<

I would use immersion oil on the camera lenses. 

-- 
______________
Bill Blank, MD
Heartland Lab

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 15
Date: Mon, 4 Aug 2008 10:20:35 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] tissue falling out of paraffin block
To: "'Price, Tiffany'" <Tiffany.Price <@t> thomaswv.org>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<mailman.2.1217869200.32328.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain;	charset="us-ascii"

I see this all the time with animal tissue, I think it is in samples that
are particularly bloody after soaking in the ice water bath before cutting,
it is like the paraffin does not infiltrate the tissue very well and it is
separate from the surrounding paraffin in the block.  Usually reembedding
does the trick for me.
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
pruegg <@t> ihctech.net
www.ihctech.net
www.ihcrg.org    

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Price,
Tiffany
Sent: Monday, August 04, 2008 7:57 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] tissue falling out of paraffin block

Can anyone tell me what would make liver tissue fall out of a paraffin
block after routine processing? We cut a routine H&E slide, and later,
had to cut IHC stains that were ordered. The tissue was fine for the
H&E, but the whole tissue fell out of the block when we tried to re-cut
it, even after re-embedding. 

Thanks for any help
Tiffany
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------------------------------

Message: 16
Date: Mon, 4 Aug 2008 12:23:03 -0400
From: "Bonner, Janet" <Janet.Bonner <@t> FLHOSP.ORG>
Subject: RE: [Histonet] stainless steel bucket
To: "Amber McKenzie" <amber.mckenzie <@t> gastrodocs.net>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID:

<5F31F38C96781A4FBE3196EBC22D47807F2748 <@t> fhosxchmb006.ADVENTISTCORP.NET>

Content-Type: text/plain; charset=iso-8859-1

Ask your surgery Department for a catalog or maybe they have some old pieces
they'd be happy to let you have!  If you're a Reference Lab with no Surgery,
Mopec carries a nice line of Pathology Morgue Equipment.
       Janet

Janet L. Bonner, HTL (ASCP)
Pathology Laboratory
Florida Hospital Winter Park
janet.bonner <@t> FLHOSP.org <mailto:janet.bonner <@t> FLHOSP.org> 
407-646-7559

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Amber McKenzie
Sent: Mon 8/4/2008 12:06 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] stainless steel bucket

Does anyone know where I can purchase a stainless steel basket/bucket to
wash my cassette lids in?  We use xylene and Liqui-Nox to wash them each
day and our plastic buckets eventually end up tearing and breaking over
time and I have to constantly replace them, so I'm looking for something
more stable like stainless steel.

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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------------------------------

Message: 17
Date: Mon, 4 Aug 2008 09:25:07 -0700
From: "R C" <ruebenjcarter <@t> gmail.com>
Subject: [Histonet] Re: Rabbit IgG control
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<2a926e3f0808040925s38e922f4mda2156f97b5592d9 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Jackson Immuno Research.

On Sat, Aug 2, 2008 at 10:01 AM,
<histonet-request <@t> lists.utsouthwestern.edu>wrote:

> Send Histonet mailing list submissions to
>        histonet <@t> lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
>        http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
>        histonet-request <@t> lists.utsouthwestern.edu
>
> You can reach the person managing the list at
>        histonet-owner <@t> lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
>   1. Rabbit IgG control (Margaryan, Naira)
>   2. Alginate beads containing cells (Danielson, Keith)
>   3. HT schools (Amber McKenzie)
>   4. Christopher Hayden is nowhere near the lab.
>      (christopher.hayden <@t> novartis.com)
>   5. A question for BondMax users (Victoria Baker)
>   6. Re: HT schools (Victoria Baker)
>   7. RE: A question for BondMax users (Beckham, Sharon)
>   8. Bone Marrow Biopsy Needles (Dana Brewer)
>   9. Re: HT schools (Rene J Buesa)
>  10. processing PFA fixed alginate beads (Gayle Callis)
>  11. Methodology for extracting nucleic acids from FFPE Tissue
>      (Gayle Callis)
>  12. RE: Dictation systems (Michael Mihalik)
>  13. Re: HT schools (Joe Nocito)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 1 Aug 2008 12:03:09 -0500
> From: "Margaryan, Naira" <NMargaryan <@t> childrensmemorial.org>
> Subject: [Histonet] Rabbit IgG control
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>        <
> EE5AF506236CEE4CAFD1A281F5DCFE90021035C8 <@t> CMHEXC02EVS.childrensmemorial.org
> >
>
> Content-Type: text/plain;       charset="us-ascii"
>
> Hi Dears,
>
>
>
> I have to order a Rabbit IgG control. What company is making best Rabbit
> IgG control, not universal, please.
>
>
>
> Thanks in advance,
>
> Naira
>
>
>
> Naira V. Margaryan, D.V.M., Ph.D.
>
> Research Scientist
>
> Children's Memorial Research Center
>
> 2300 Children's Plaza, Box 222
>
> Chicago, IL 60614-3363
>
> Tel: 773-755-6340
>
> Fax: 773-755-6594
>
> nmargaryan <@t> childrensmemorial.org
> <mailto:nmargaryan <@t> childrensmemorial.org>
>
>
>
> For Express Mail:
>
> CMRC, Room C.473
>
> 2430 N. Halsted Street
>
> Chicago, IL  60614-4314
>
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Fri, 1 Aug 2008 13:07:21 -0400
> From: "Danielson, Keith" <Keith.Danielson <@t> uphs.upenn.edu>
> Subject: [Histonet] Alginate beads containing cells
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
>  <84D11048BB18234D8A56474645E8EC020829EF6F <@t> uphsmbx5.UPHS.PENNHEALTH.PRV>
>
> Content-Type: text/plain;       charset="US-ASCII"
>
> Hello everyone,
>
>
>
> I am requesting information for a graduate student on how to fix and
> process alginate beads with embedded tissue culture cells for paraffin
> histology. The beads are fixed in 4% paraformaldehyde but seem to turn
> white and disintegrate during routine processing.
>
>
>
> Thanks very much,
>
>
>
> Keith Danielson, PhD
>
> Supervisor, IHC Lab
>
> Pennsylvania Hospital
>
> Philadelphia, PA 19107
>
>
>
> Phone: 215 829-7726 or 829-3696
>
> E-mail: keith.danielson <@t> uphs.upenn.edu
>
>
>
>
>
>
>
> The information contained in this e-mail message is intended only for the
> personal and confidential use of the recipient(s) named above. If the
reader
> of this message is not the intended recipient or an agent responsible for
> delivering it to the intended recipient, you are hereby notified that you
> have received this document in error and that any review, dissemination,
> distribution, or copying of this message is strictly prohibited. If you
have
> received this communication in error, please notify us immediately by
> e-mail, and delete the original message.
>
> ------------------------------
>
> Message: 3
> Date: Fri, 1 Aug 2008 12:23:42 -0500
> From: "Amber McKenzie" <amber.mckenzie <@t> gastrodocs.net>
> Subject: [Histonet] HT schools
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>        <03C921A1EAF7F541B16543F6EC6A4B3701D3AEBF <@t> giamail2.Gia.com>
> Content-Type: text/plain;       charset="US-ASCII"
>
> Where are all the HT accredited schools and why aren't there more out
> there?  I've seen the online classes' people can take, but that requires
> them to be trained in a lab, as well, for the "hands on" part.  So,
> actually the supervisor still has to train potential HT's "on the job"
> before they can sit for the board exam.  Right?
>
>
>
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Fri, 1 Aug 2008 13:41:00 -0400
> From: christopher.hayden <@t> novartis.com
> Subject: [Histonet] Christopher Hayden is nowhere near the lab.
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
>        <
> OFC6859B0A.C0BE57E7-ON85257498.0061233C-85257498.0061233C <@t> ah.novartis.com>
>
> Content-Type: text/plain; charset=US-ASCII
>
>
> I will be out of the office starting  08/01/2008 and will not return until
> 08/11/2008.
>
> Good Day Everyone!
>
> I will be out of the lab from 2 August  until 10 August. I'll be back at
my
> desk on the morning of the 11th. I will have no access to corporate email
> during this time.
>
> If you have an EM-related matter, please contact the Lab Manager, Gregory
> Argentieri, at x2-8617
>
> Otherwise, I'll get back to you on the 11th or shortly afterwards.
>
> Thanks!
> -CH
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Fri, 1 Aug 2008 14:13:13 -0400
> From: "Victoria Baker" <bakevictoria <@t> gmail.com>
> Subject: [Histonet] A question for BondMax users
> To: "Histo Net list server" <HistoNet <@t> lists.utsouthwestern.edu>
> Message-ID:
>        <4f016b690808011113k4bceed66w3a76c8bb38fa8cb <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Happy Friday everyone!
>
> In the BondMax detection system there is a "post primary" step that
> contains 10% BSA in a Tris buffer.  I've tested the protocol excluding
> this step and did not have any staining.
>
> I can't seem to get the answer I'm looking for from the company, so if
> anyone can help me I'd really appreciate it.
>
> Thanks
>
> Vikki
>
>
>
> ------------------------------
>
> Message: 6
> Date: Fri, 1 Aug 2008 14:18:43 -0400
> From: "Victoria Baker" <bakevictoria <@t> gmail.com>
> Subject: Re: [Histonet] HT schools
> To: "Amber McKenzie" <amber.mckenzie <@t> gastrodocs.net>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
>        <4f016b690808011118k34ecf6c5r20c4960d00b679c6 <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi Amber,
>
> If you go to the NSH website there are schools listed there.  I'm an
> alumni of the SUNY school at Cobleskill - and I loved it - but it was
> also thirty years ago.
>
> I would check that list and see if you can't make some calls or
> e-mails to their co-ordinators.
>
> Regards,
>
> Vikki Baker
>
>
>
> On 8/1/08, Amber McKenzie <amber.mckenzie <@t> gastrodocs.net> wrote:
> > Where are all the HT accredited schools and why aren't there more out
> > there?  I've seen the online classes' people can take, but that requires
> > them to be trained in a lab, as well, for the "hands on" part.  So,
> > actually the supervisor still has to train potential HT's "on the job"
> > before they can sit for the board exam.  Right?
> >
> >
> >
> >
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
>
> ------------------------------
>
> Message: 7
> Date: Fri, 1 Aug 2008 13:27:36 -0500
> From: "Beckham, Sharon" <SLB <@t> Stowers-Institute.org>
> Subject: RE: [Histonet] A question for BondMax users
> To: 'Victoria Baker' <bakevictoria <@t> gmail.com>, Histo Net list server
>        <HistoNet <@t> lists.utsouthwestern.edu>
> Message-ID:
>        <
> BD62CBAC4395B94096109020651BE2EC129EDDCE2F <@t> exchmb-02.stowers-institute.org
> >
>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi Vikki,
>
> I don't know if I'm much help on this because I just started testing the
> BondMax this week so I'm just in the learning process too.  As far as I
can
> tell the post primary is for blocking, but also works in conjunction with
> the polymer which is the following step.  It supposedly also enhances
> penetration of the polymer, so they both have to be used together.  Hope
> this helps.
>
> Sharon
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Victoria Baker
> Sent: Friday, August 01, 2008 1:13 PM
> To: Histo Net list server
> Subject: [Histonet] A question for BondMax users
>
>
> Happy Friday everyone!
>
> In the BondMax detection system there is a "post primary" step that
> contains 10% BSA in a Tris buffer.  I've tested the protocol excluding
this
> step and did not have any staining.
>
> I can't seem to get the answer I'm looking for from the company, so if
> anyone can help me I'd really appreciate it.
>
> Thanks
>
> Vikki
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 8
> Date: Fri, 1 Aug 2008 13:27:28 -0500
> From: "Dana Brewer" <brewerd <@t> nacmem.org>
> Subject: [Histonet] Bone Marrow Biopsy Needles
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <000601c8f404$619b66c0$0b0a10ac <@t> work.nmh.org>
> Content-Type: text/plain;       charset="iso-8859-1"
>
> Does anyone have a replacement source for J Style Needle, -Marrow Loc-
Bone
> Marrow Biopsy Needles -- no longer manufactured by US BIOPSY -- the
> replacement we got is not the same "marrow loc" system our oncologist
> prefers.
> Thanks for your help
>
> ------------------------------
>
> Message: 9
> Date: Fri, 1 Aug 2008 12:42:14 -0700 (PDT)
> From: Rene J Buesa <rjbuesa <@t> yahoo.com>
> Subject: Re: [Histonet] HT schools
> To: histonet <@t> lists.utsouthwestern.edu,  Amber McKenzie
>        <amber.mckenzie <@t> gastrodocs.net>
> Message-ID: <78031.12752.qm <@t> web65716.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Wrong! The advantage of the "on line" or "distance learning" courses is
> that they provide the theory on line while you are working at a given
> laboratory doing your training (or even as part of your daily work) so
there
> is no "actual training" to be done by the supervisor.
> At this moment NAACLS has 30 HT and 3 HTL accredited programs only, with
an
> overall capacity of about 300-325 students, and this will not be enough to
> take care of  all the retiring histotechs.
> Costs is one of the reasons why the number of HTs schools is dwindling.
> Reni J.
>
>
> --- On Fri, 8/1/08, Amber McKenzie <amber.mckenzie <@t> gastrodocs.net> wrote:
>
> From: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>
> Subject: [Histonet] HT schools
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Friday, August 1, 2008, 1:23 PM
>
> Where are all the HT accredited schools and why aren't there more out
> there?  I've seen the online classes' people can take, but that
> requires
> them to be trained in a lab, as well, for the "hands on" part.  So,
> actually the supervisor still has to train potential HT's "on the
> job"
> before they can sit for the board exam.  Right?
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 10
> Date: Fri, 1 Aug 2008 15:23:31 -0600
> From: "Gayle Callis" <gayle.callis <@t> bresnan.net>
> Subject: [Histonet] processing PFA fixed alginate beads
> To: "Histonet Message" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <000801c8f41c$d9196180$6401a8c0 <@t> Sunney>
> Content-Type: text/plain;       charset="iso-8859-1"
>
> I Googled this subject and brought up several publications using alginate
> beads embedded in paraffin. HOwever, the following publication, using
> cryotomy, had better results than using paraffin sections.
>
> Also, Yang C-C, et al.  Adapted cyrosectioning method for hydrogels used
in
> regenerative medicine.  J Histotechnology 30(3):185-191, 2007.  Linda
> Jenkins, on of the co-authors, was a huge part of this, has much
experience
> with hydrogels.   In this publication, they fabricated their own alginate
> beads.   The reference list is extensive and informative.
>
> Good Luck
>
> Gayle M. Callis
> HTL/HT/MT(ASCP)
>
>
>
>
----------------------------------------------------------------------------
----
>
>
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Fri, 1 Aug 2008 15:28:53 -0600
> From: "Gayle Callis" <gayle.callis <@t> bresnan.net>
> Subject: [Histonet] Methodology for extracting nucleic acids from FFPE
>        Tissue
> To: "Histonet Message" <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <000f01c8f41d$990a30f0$6401a8c0 <@t> Sunney>
> Content-Type: text/plain;       charset="iso-8859-1"
>
> For the person needing a protocol for extracting DNA and/or RNA from
breast
> (?) tissue, check out QuickExtract FFPE DNA and RNA Extraction Kits from
> Epicenter Biotechnologies,    www. EpiBio.com
>
> Phone # is 800 284-8474.
>
> This information was found in the latest issue of Science ( Life ScienCe
> Technologies section)
>
> Gayle M. Callis
> HTL/HT/MT(ASCP)
>
>
>
> ------------------------------
>
> Message: 12
> Date: Fri, 1 Aug 2008 18:14:56 -0400
> From: "Michael Mihalik" <mike <@t> pathview.com>
> Subject: RE: [Histonet] Dictation systems
> To: <ht.ascp <@t> yahoo.com>,        <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <001501c8f424$204a2830$60de7890$@com>
> Content-Type: text/plain;       charset="iso-8859-1"
>
> Jon, did you ever get an answer to this question?
>
> I am not as familiar with all the various dictation systems that I would
> like to be, but I have used something that may help.
>
> You can buy a foot pedal that can be 'programmed' to send a hotkey
> combination to an external device, typically a PC.
>
> In my case,  I was considering using this setup to enable and disable
> Dragon
> Naturally Speaking at the gross workstation.
>
> Michael Mihalik
> PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jon Google
> Sent: Tuesday, July 29, 2008 3:29 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Dictation systems
>
> We are in the process of upgrading our LIS. We are looking at moving our
> dictation system from our current Dictaphone analog system. We would like
> to
> use a digital system that can support VOIP.  The problem we are running
> into
> is trying to find one that supports a hands free control that our grossing
> pathologists need.
>
> Does anyone have recommendations on digital systems that support foot
> controls?
>
> Thanks
> Jon
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 13
> Date: Sat, 2 Aug 2008 07:19:36 -0500
> From: "Joe Nocito" <jnocito <@t> satx.rr.com>
> Subject: Re: [Histonet] HT schools
> To: <rjbuesa <@t> yahoo.com>, <histonet <@t> lists.utsouthwestern.edu>,   "Amber
>        McKenzie" <amber.mckenzie <@t> gastrodocs.net>
> Message-ID: <002c01c8f49a$07995790$e797b348 <@t> yourxhtr8hvc4p>
> Content-Type: text/plain; format=flowed; charset="iso-8859-1";
>        reply-type=original
>
> our one community college program in San Antonio is in jeopardy of closing
> because it can't get the required 10 students to make a class. If this
> program does close, I will be willing to work with a college or university
> with long- distance learning. I have 5 openings in my lab.
>
> JTT
> ----- Original Message -----
> From: "Rene J Buesa" <rjbuesa <@t> yahoo.com>
> To: <histonet <@t> lists.utsouthwestern.edu>; "Amber McKenzie"
> <amber.mckenzie <@t> gastrodocs.net>
> Sent: Friday, August 01, 2008 2:42 PM
> Subject: Re: [Histonet] HT schools
>
>
> Wrong! The advantage of the "on line" or "distance learning" courses is
> that
> they provide the theory on line while you are working at a given
laboratory
> doing your training (or even as part of your daily work) so there is no
> "actual training" to be done by the supervisor.
> At this moment NAACLS has 30 HT and 3 HTL accredited programs only, with
an
> overall capacity of about 300-325 students, and this will not be enough to
> take care of all the retiring histotechs.
> Costs is one of the reasons why the number of HTs schools is dwindling.
> Reni J.
>
>
> --- On Fri, 8/1/08, Amber McKenzie <amber.mckenzie <@t> gastrodocs.net> wrote:
>
> From: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>
> Subject: [Histonet] HT schools
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Friday, August 1, 2008, 1:23 PM
>
> Where are all the HT accredited schools and why aren't there more out
> there?  I've seen the online classes' people can take, but that
> requires
> them to be trained in a lab, as well, for the "hands on" part.  So,
> actually the supervisor still has to train potential HT's "on the
> job"
> before they can sit for the board exam.  Right?
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 57, Issue 2
> ***************************************
>

------------------------------

Message: 18
Date: Mon, 4 Aug 2008 10:46:26 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] HT schools
To: "'Cheri Miller'" <cmiller <@t> physlab.com>, <rjbuesa <@t> yahoo.com>,
	<histonet <@t> lists.utsouthwestern.edu>,	"'Amber McKenzie'"
	<amber.mckenzie <@t> gastrodocs.net>
Message-ID:
	<mailman.3.1217869200.32328.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain;	charset="iso-8859-1"

Remember that now there is no practical portion of the HT exam, so they are
not being tested on hands on experiences anyway.  The most difficult problem
I have with training people on the job (and I have trained many) is that now
they are not prepared to take the exam because they are examined all on
theory.  I have some really well trained people who can do the work really
well, but they have a hard time taking the computer test which they pretty
much have to memorize out of books.
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
pruegg <@t> ihctech.net
www.ihctech.net
www.ihcrg.org    

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheri Miller
Sent: Monday, August 04, 2008 7:05 AM
To: rjbuesa <@t> yahoo.com; histonet <@t> lists.utsouthwestern.edu; 'Amber McKenzie'
Subject: RE: [Histonet] HT schools

Someone has to teach them the "hands on" part of histology. I do not leave
this to my staff. I teach / give them the skills they need to perform the
practical part of their profession. As their supervisor I am very much
involved in their training. I am sure I am not the only one.??

Cheryl Miller HT (ASCP)
Histology Supervisor
Physicians Laboratory,P.C.
Omaha, Ne. 
402 738 5052

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, August 01, 2008 2:42 PM
To: histonet <@t> lists.utsouthwestern.edu; Amber McKenzie
Subject: Re: [Histonet] HT schools

Wrong! The advantage of the "on line" or "distance learning" courses is that
they provide the theory on line while you are working at a given laboratory
doing your training (or even as part of your daily work) so there is no
"actual training" to be done by the supervisor.
At this moment NAACLS has 30 HT and 3 HTL accredited programs only, with an
overall capacity of about 300-325 students, and this will not be enough to
take care of  all the retiring histotechs.
Costs is one of the reasons why the number of HTs schools is dwindling.
Reni J.

--- On Fri, 8/1/08, Amber McKenzie <amber.mckenzie <@t> gastrodocs.net> wrote:

From: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>
Subject: [Histonet] HT schools
To: histonet <@t> lists.utsouthwestern.edu
Date: Friday, August 1, 2008, 1:23 PM

Where are all the HT accredited schools and why aren't there more out
there?  I've seen the online classes' people can take, but that
requires
them to be trained in a lab, as well, for the "hands on" part.  So,
actually the supervisor still has to train potential HT's "on the
job"
before they can sit for the board exam.  Right? 

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------------------------------

Message: 19
Date: Mon, 4 Aug 2008 10:46:26 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] surveillance cameras in the lab
To: "'Cheri Miller'" <cmiller <@t> physlab.com>, <Pathrm35 <@t> comcast.net>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<mailman.4.1217869200.32328.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain;	charset="us-ascii"

Come on.
In these times of terror concerns I am not sure I would work in a place
where I did not feel secure and the use of these devices help in that
matter.
We built a brand new University of Colorado Health Sciences Center and there
are cameras all over the place as well as lock down.  If you do not have an
access card you cannot get into the labs.  This was a pain at first but with
all the crazy's we have to worry about out there it now makes me feel
better.  
I just read in the paper this morning about a researcher whose house was
bombed by Peta types for doing animal research, and we have had all sorts of
disturbances over the years with precious research animals being released,
protests, etc.
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
pruegg <@t> ihctech.net
www.ihctech.net
www.ihcrg.org    

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheri Miller
Sent: Monday, August 04, 2008 7:08 AM
To: Pathrm35 <@t> comcast.net; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] surveillance cameras in the lab

That is B.S....I wouldn't work in a lab that used covalence camera's for
what ever reason

Cheryl Miller HT (ASCP)
Histology Supervisor
Physicians Laboratory,P.C.
Omaha, Ne. 
402 738 5052

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Pathrm35 <@t> comcast.net
Sent: Monday, August 04, 2008 5:21 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] surveillance cameras in the lab

I was wondering how many techs out there have cameras in their labs, either
for security or to monitor employees. I went to work Sunday night and
noticed that 4 cameras were installed in the lab over the weekend, with more
to come.
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PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.  If
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------------------------------

Message: 20
Date: Mon, 4 Aug 2008 12:58:44 -0400
From: "William Connor" <will <@t> histologytechservices.com>
Subject: [Histonet] QIHC Certification
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <0MKpCa-1KQ3OY3GeG-0004hL <@t> mrelay.perfora.net>
Content-Type: text/plain;	charset="us-ascii"

Greetings,

I have just sent in my application in for the QIHC exam.  Would anyone out
there kindly recommend to me the best study materials to focus on?  I have
Carson's book and Dako's Immunohistochemical Staining Methods, and am
considering the purchase of Diagnostic Immunohistochemistry by Dabbs.  I do
not work in a hospital setting, but in more of a research setting.  Would
there be anything I might be missing out on in day to day practice which
might be covered on the exam?

Thank you in advance for your responses.

William P. Connor, HT(ASCP)

Senior Histology Technician

Histology Tech Services, Inc.

This email and its attachments (if any) contain confidential and privileged
information.  Any dissemination or use of this information by a person other
than the intended recipient is unauthorized and may be illegal.

------------------------------

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End of Histonet Digest, Vol 57, Issue 5
***************************************