[Histonet] tissue falling out of paraffin block

Patsy Ruegg pruegg <@t> ihctech.net
Mon Aug 4 11:20:35 CDT 2008

I see this all the time with animal tissue, I think it is in samples that
are particularly bloody after soaking in the ice water bath before cutting,
it is like the paraffin does not infiltrate the tissue very well and it is
separate from the surrounding paraffin in the block.  Usually reembedding
does the trick for me.

Patsy Ruegg, HT(ASCP)QIHC
12635 Montview Blvd. #215
Aurora, CO 80045
fax 720-859-4110
pruegg <@t> ihctech.net

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Price,
Sent: Monday, August 04, 2008 7:57 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] tissue falling out of paraffin block

Can anyone tell me what would make liver tissue fall out of a paraffin
block after routine processing? We cut a routine H&E slide, and later,
had to cut IHC stains that were ordered. The tissue was fine for the
H&E, but the whole tissue fell out of the block when we tried to re-cut
it, even after re-embedding. 
Thanks for any help
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