[Histonet] tissue falling out of paraffin block

[Patsy Ruegg]

I see this all the time with animal tissue, I think it is in samples that
are particularly bloody after soaking in the ice water bath before cutting,
it is like the paraffin does not infiltrate the tissue very well and it is
separate from the surrounding paraffin in the block.  Usually reembedding
does the trick for me.
Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. #215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
pruegg <@t> ihctech.net
www.ihctech.net
www.ihcrg.org    
 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Price,
Tiffany
Sent: Monday, August 04, 2008 7:57 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] tissue falling out of paraffin block

Can anyone tell me what would make liver tissue fall out of a paraffin
block after routine processing? We cut a routine H&E slide, and later,
had to cut IHC stains that were ordered. The tissue was fine for the
H&E, but the whole tissue fell out of the block when we tried to re-cut
it, even after re-embedding. 
 
Thanks for any help
Tiffany
Confidentiality Notice:   This email message including any attachments, is
for the sole use of the intended recipient(s) and may contain confidential
and privilege information.   Any unauthorized review, use, disclosure or
distribution is prohibited.   If you are not the intended recipient, please
contact the sender by reply email and destroy ALL copies of the message.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet