[Histonet] RE: A question for BondMax users [Scanned]

[Sheila MacPherson]

We have been using a Bond immunostaining system for several years now.  The pre polymer is a rabbit anti mouse linker, to allow the detection of either rabbit or mouse antibodies.  The polymer is anti rabbit so if you are using  a mouse primary you will need the pre polymer and for a  rabbit primary the pre polymer can be edited out of the protocol (can reduce background if it is a problem)  For goat primaries we substitute a rabbit anti goat secondary link instead of the pre polymer.
Sheila

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: 02 August 2008 18:06
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 57, Issue 2 [Scanned]

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Today's Topics:

   1. Rabbit IgG control (Margaryan, Naira)
   2. Alginate beads containing cells (Danielson, Keith)
   3. HT schools (Amber McKenzie)
   4. Christopher Hayden is nowhere near the lab.
      (christopher.hayden <@t> novartis.com)
   5. A question for BondMax users (Victoria Baker)
   6. Re: HT schools (Victoria Baker)
   7. RE: A question for BondMax users (Beckham, Sharon)
   8. Bone Marrow Biopsy Needles (Dana Brewer)
   9. Re: HT schools (Rene J Buesa)
  10. processing PFA fixed alginate beads (Gayle Callis)
  11. Methodology for extracting nucleic acids from FFPE Tissue
      (Gayle Callis)
  12. RE: Dictation systems (Michael Mihalik)
  13. Re: HT schools (Joe Nocito)


----------------------------------------------------------------------

Message: 1
Date: Fri, 1 Aug 2008 12:03:09 -0500
From: "Margaryan, Naira" <NMargaryan <@t> childrensmemorial.org>
Subject: [Histonet] Rabbit IgG control
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<EE5AF506236CEE4CAFD1A281F5DCFE90021035C8 <@t> CMHEXC02EVS.childrensmemorial.org>
	
Content-Type: text/plain;	charset="us-ascii"

Hi Dears,

 

I have to order a Rabbit IgG control. What company is making best Rabbit
IgG control, not universal, please.

 

Thanks in advance,

Naira

 

Naira V. Margaryan, D.V.M., Ph.D.

Research Scientist

Children's Memorial Research Center

2300 Children's Plaza, Box 222

Chicago, IL 60614-3363

Tel: 773-755-6340

Fax: 773-755-6594

nmargaryan <@t> childrensmemorial.org
<mailto:nmargaryan <@t> childrensmemorial.org> 

 

For Express Mail: 

CMRC, Room C.473

2430 N. Halsted Street

Chicago, IL  60614-4314

 



------------------------------

Message: 2
Date: Fri, 1 Aug 2008 13:07:21 -0400
From: "Danielson, Keith" <Keith.Danielson <@t> uphs.upenn.edu>
Subject: [Histonet] Alginate beads containing cells
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<84D11048BB18234D8A56474645E8EC020829EF6F <@t> uphsmbx5.UPHS.PENNHEALTH.PRV>
	
Content-Type: text/plain;	charset="US-ASCII"

Hello everyone,

 

I am requesting information for a graduate student on how to fix and
process alginate beads with embedded tissue culture cells for paraffin
histology. The beads are fixed in 4% paraformaldehyde but seem to turn
white and disintegrate during routine processing. 

 

Thanks very much,

 

Keith Danielson, PhD

Supervisor, IHC Lab

Pennsylvania Hospital

Philadelphia, PA 19107

 

Phone: 215 829-7726 or 829-3696

E-mail: keith.danielson <@t> uphs.upenn.edu

 

 



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------------------------------

Message: 3
Date: Fri, 1 Aug 2008 12:23:42 -0500
From: "Amber McKenzie" <amber.mckenzie <@t> gastrodocs.net>
Subject: [Histonet] HT schools
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<03C921A1EAF7F541B16543F6EC6A4B3701D3AEBF <@t> giamail2.Gia.com>
Content-Type: text/plain;	charset="US-ASCII"

Where are all the HT accredited schools and why aren't there more out
there?  I've seen the online classes' people can take, but that requires
them to be trained in a lab, as well, for the "hands on" part.  So,
actually the supervisor still has to train potential HT's "on the job"
before they can sit for the board exam.  Right? 

 

 



------------------------------

Message: 4
Date: Fri, 1 Aug 2008 13:41:00 -0400
From: christopher.hayden <@t> novartis.com
Subject: [Histonet] Christopher Hayden is nowhere near the lab.
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<OFC6859B0A.C0BE57E7-ON85257498.0061233C-85257498.0061233C <@t> ah.novartis.com>
	
Content-Type: text/plain; charset=US-ASCII


I will be out of the office starting  08/01/2008 and will not return until
08/11/2008.

Good Day Everyone!

I will be out of the lab from 2 August  until 10 August. I'll be back at my
desk on the morning of the 11th. I will have no access to corporate email
during this time.

If you have an EM-related matter, please contact the Lab Manager, Gregory
Argentieri, at x2-8617

Otherwise, I'll get back to you on the 11th or shortly afterwards.

Thanks!
-CH




------------------------------

Message: 5
Date: Fri, 1 Aug 2008 14:13:13 -0400
From: "Victoria Baker" <bakevictoria <@t> gmail.com>
Subject: [Histonet] A question for BondMax users
To: "Histo Net list server" <HistoNet <@t> lists.utsouthwestern.edu>
Message-ID:
	<4f016b690808011113k4bceed66w3a76c8bb38fa8cb <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Happy Friday everyone!

In the BondMax detection system there is a "post primary" step that
contains 10% BSA in a Tris buffer.  I've tested the protocol excluding
this step and did not have any staining.

I can't seem to get the answer I'm looking for from the company, so if
anyone can help me I'd really appreciate it.

Thanks

Vikki



------------------------------

Message: 6
Date: Fri, 1 Aug 2008 14:18:43 -0400
From: "Victoria Baker" <bakevictoria <@t> gmail.com>
Subject: Re: [Histonet] HT schools
To: "Amber McKenzie" <amber.mckenzie <@t> gastrodocs.net>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<4f016b690808011118k34ecf6c5r20c4960d00b679c6 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hi Amber,

If you go to the NSH website there are schools listed there.  I'm an
alumni of the SUNY school at Cobleskill - and I loved it - but it was
also thirty years ago.

I would check that list and see if you can't make some calls or
e-mails to their co-ordinators.

Regards,

Vikki Baker



On 8/1/08, Amber McKenzie <amber.mckenzie <@t> gastrodocs.net> wrote:
> Where are all the HT accredited schools and why aren't there more out
> there?  I've seen the online classes' people can take, but that requires
> them to be trained in a lab, as well, for the "hands on" part.  So,
> actually the supervisor still has to train potential HT's "on the job"
> before they can sit for the board exam.  Right?
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



------------------------------

Message: 7
Date: Fri, 1 Aug 2008 13:27:36 -0500
From: "Beckham, Sharon" <SLB <@t> Stowers-Institute.org>
Subject: RE: [Histonet] A question for BondMax users
To: 'Victoria Baker' <bakevictoria <@t> gmail.com>, Histo Net list server
	<HistoNet <@t> lists.utsouthwestern.edu>
Message-ID:
	<BD62CBAC4395B94096109020651BE2EC129EDDCE2F <@t> exchmb-02.stowers-institute.org>
	
Content-Type: text/plain; charset="us-ascii"

Hi Vikki,

I don't know if I'm much help on this because I just started testing the BondMax this week so I'm just in the learning process too.  As far as I can tell the post primary is for blocking, but also works in conjunction with the polymer which is the following step.  It supposedly also enhances penetration of the polymer, so they both have to be used together.  Hope this helps.

Sharon


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Victoria Baker
Sent: Friday, August 01, 2008 1:13 PM
To: Histo Net list server
Subject: [Histonet] A question for BondMax users


Happy Friday everyone!

In the BondMax detection system there is a "post primary" step that contains 10% BSA in a Tris buffer.  I've tested the protocol excluding this step and did not have any staining.

I can't seem to get the answer I'm looking for from the company, so if anyone can help me I'd really appreciate it.

Thanks

Vikki

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 8
Date: Fri, 1 Aug 2008 13:27:28 -0500
From: "Dana Brewer" <brewerd <@t> nacmem.org>
Subject: [Histonet] Bone Marrow Biopsy Needles
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000601c8f404$619b66c0$0b0a10ac <@t> work.nmh.org>
Content-Type: text/plain;	charset="iso-8859-1"

Does anyone have a replacement source for J Style Needle, -Marrow Loc- Bone Marrow Biopsy Needles -- no longer manufactured by US BIOPSY -- the replacement we got is not the same "marrow loc" system our oncologist prefers.  
Thanks for your help 

------------------------------

Message: 9
Date: Fri, 1 Aug 2008 12:42:14 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] HT schools
To: histonet <@t> lists.utsouthwestern.edu,	Amber McKenzie
	<amber.mckenzie <@t> gastrodocs.net>
Message-ID: <78031.12752.qm <@t> web65716.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Wrong! The advantage of the "on line" or "distance learning" courses is that they provide the theory on line while you are working at a given laboratory doing your training (or even as part of your daily work) so there is no "actual training" to be done by the supervisor.
At this moment NAACLS has 30 HT and 3 HTL accredited programs only, with an overall capacity of about 300-325 students, and this will not be enough to take care of  all the retiring histotechs.
Costs is one of the reasons why the number of HTs schools is dwindling.
René J.
 

--- On Fri, 8/1/08, Amber McKenzie <amber.mckenzie <@t> gastrodocs.net> wrote:

From: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>
Subject: [Histonet] HT schools
To: histonet <@t> lists.utsouthwestern.edu
Date: Friday, August 1, 2008, 1:23 PM

Where are all the HT accredited schools and why aren't there more out
there?  I've seen the online classes' people can take, but that
requires
them to be trained in a lab, as well, for the "hands on" part.  So,
actually the supervisor still has to train potential HT's "on the
job"
before they can sit for the board exam.  Right? 

 

 

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      

------------------------------

Message: 10
Date: Fri, 1 Aug 2008 15:23:31 -0600
From: "Gayle Callis" <gayle.callis <@t> bresnan.net>
Subject: [Histonet] processing PFA fixed alginate beads
To: "Histonet Message" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000801c8f41c$d9196180$6401a8c0 <@t> Sunney>
Content-Type: text/plain;	charset="iso-8859-1"

I Googled this subject and brought up several publications using alginate beads embedded in paraffin. HOwever, the following publication, using cryotomy, had better results than using paraffin sections.  

Also, Yang C-C, et al.  Adapted cyrosectioning method for hydrogels used in regenerative medicine.  J Histotechnology 30(3):185-191, 2007.  Linda Jenkins, on of the co-authors, was a huge part of this, has much experience with hydrogels.   In this publication, they fabricated their own alginate beads.   The reference list is extensive and informative. 

Good Luck

Gayle M. Callis
HTL/HT/MT(ASCP)


--------------------------------------------------------------------------------




 

------------------------------

Message: 11
Date: Fri, 1 Aug 2008 15:28:53 -0600
From: "Gayle Callis" <gayle.callis <@t> bresnan.net>
Subject: [Histonet] Methodology for extracting nucleic acids from FFPE
	Tissue
To: "Histonet Message" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000f01c8f41d$990a30f0$6401a8c0 <@t> Sunney>
Content-Type: text/plain;	charset="iso-8859-1"

For the person needing a protocol for extracting DNA and/or RNA from breast (?) tissue, check out QuickExtract FFPE DNA and RNA Extraction Kits from Epicenter Biotechnologies,    www. EpiBio.com   

Phone # is 800 284-8474. 

This information was found in the latest issue of Science ( Life ScienCe Technologies section) 

Gayle M. Callis
HTL/HT/MT(ASCP) 



------------------------------

Message: 12
Date: Fri, 1 Aug 2008 18:14:56 -0400
From: "Michael Mihalik" <mike <@t> pathview.com>
Subject: RE: [Histonet] Dictation systems
To: <ht.ascp <@t> yahoo.com>,	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001501c8f424$204a2830$60de7890$@com>
Content-Type: text/plain;	charset="iso-8859-1"

Jon, did you ever get an answer to this question?  

I am not as familiar with all the various dictation systems that I would
like to be, but I have used something that may help.

You can buy a foot pedal that can be 'programmed' to send a hotkey
combination to an external device, typically a PC.

In my case,  I was considering using this setup to enable and disable Dragon
Naturally Speaking at the gross workstation.

Michael Mihalik
PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 270.423.0968
 
 
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jon Google
Sent: Tuesday, July 29, 2008 3:29 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Dictation systems

We are in the process of upgrading our LIS. We are looking at moving our
dictation system from our current Dictaphone analog system. We would like to
use a digital system that can support VOIP.  The problem we are running into
is trying to find one that supports a hands free control that our grossing
pathologists need.
 
Does anyone have recommendations on digital systems that support foot
controls?
 
Thanks
Jon


      
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 13
Date: Sat, 2 Aug 2008 07:19:36 -0500
From: "Joe Nocito" <jnocito <@t> satx.rr.com>
Subject: Re: [Histonet] HT schools
To: <rjbuesa <@t> yahoo.com>, <histonet <@t> lists.utsouthwestern.edu>,	"Amber
	McKenzie" <amber.mckenzie <@t> gastrodocs.net>
Message-ID: <002c01c8f49a$07995790$e797b348 <@t> yourxhtr8hvc4p>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

our one community college program in San Antonio is in jeopardy of closing 
because it can't get the required 10 students to make a class. If this 
program does close, I will be willing to work with a college or university 
with long- distance learning. I have 5 openings in my lab.

JTT
----- Original Message ----- 
From: "Rene J Buesa" <rjbuesa <@t> yahoo.com>
To: <histonet <@t> lists.utsouthwestern.edu>; "Amber McKenzie" 
<amber.mckenzie <@t> gastrodocs.net>
Sent: Friday, August 01, 2008 2:42 PM
Subject: Re: [Histonet] HT schools


Wrong! The advantage of the "on line" or "distance learning" courses is that 
they provide the theory on line while you are working at a given laboratory 
doing your training (or even as part of your daily work) so there is no 
"actual training" to be done by the supervisor.
At this moment NAACLS has 30 HT and 3 HTL accredited programs only, with an 
overall capacity of about 300-325 students, and this will not be enough to 
take care of all the retiring histotechs.
Costs is one of the reasons why the number of HTs schools is dwindling.
René J.


--- On Fri, 8/1/08, Amber McKenzie <amber.mckenzie <@t> gastrodocs.net> wrote:

From: Amber McKenzie <amber.mckenzie <@t> gastrodocs.net>
Subject: [Histonet] HT schools
To: histonet <@t> lists.utsouthwestern.edu
Date: Friday, August 1, 2008, 1:23 PM

Where are all the HT accredited schools and why aren't there more out
there?  I've seen the online classes' people can take, but that
requires
them to be trained in a lab, as well, for the "hands on" part.  So,
actually the supervisor still has to train potential HT's "on the
job"
before they can sit for the board exam.  Right?





_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 




------------------------------

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Histonet mailing list
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End of Histonet Digest, Vol 57, Issue 2
***************************************