[Histonet] processing PFA fixed alginate beads

[Gayle Callis]

I Googled this subject and brought up several publications using alginate beads embedded in paraffin. HOwever, the following publication, using cryotomy, had better results than using paraffin sections.  

Also, Yang C-C, et al.  Adapted cyrosectioning method for hydrogels used in regenerative medicine.  J Histotechnology 30(3):185-191, 2007.  Linda Jenkins, on of the co-authors, was a huge part of this, has much experience with hydrogels.   In this publication, they fabricated their own alginate beads.   The reference list is extensive and informative. 

Good Luck

Gayle M. Callis
HTL/HT/MT(ASCP)


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