[Histonet] Thymidylate Phosphostase

[Dana Settembre]

Hello,
I am looking for Thymidylate Phosphotase for use on
formalin fixed paraffin embedded human tissue.
Is anyone using this on paraffin?  What vendor do you use?
Thank you,


Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA


>>> Joe Nocito <jnocito <@t> satx.rr.com> 04/29/08 7:25 PM >>>
Joyce,
no and yes. We go through so many controls that we often exhaust the
block, 
especially on the scarce controls like AFB,  AFP, ADV.
We have the Ventana stainers also and verify the different lot numbers
also.

JTT
----- Original Message ----- 
From: "Joyce Cline" <jcline <@t> wchsys.org>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, April 29, 2008 11:00 AM
Subject: [Histonet] validation of lot #'s for IHC & Specials


> We currently pull a control already stained with the old lot # and
stain
> a control with the new lot # and compare staining. Our paths are
saying
> you should use the same control block for both the old and new lot
#'s
> but we don't always have the same block (might have been exhausted)
>
> Does anyone keep a control block without exhausting it, for lot
> validation?
> Does anyone check lot #'s for their special stains. We have a
Ventana
> NexES Sp. Stainer and we order pre-made kits for our hand staining.
Our
> paths want us to validate the lot #'s for our specials also.
>
> Thanks..Joyce :-)
>
>
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