FW: [Histonet] Staining Procedures for Technovit 7200

[Suzanne Bruce]

Thanks Gayle, 
 
I am searching for a VVG or any other special stains. I think I have found a VVG published earlier in the archives. 
 
Suzanne Bruce,  R.V.T.
Histologist 
________________________________

From: Gayle Callis [mailto:gayle.callis <@t> bresnan.net]
Sent: Sat 4/26/2008 11:03 AM
To: Suzanne Bruce
Subject: Re: [Histonet] Staining Procedures for Technovit 7200



Technovit it GMA.  If you do a search for glycol methacrylate in Histonet
website, you will find methods.  If you tell us what stains you want to do,
it will help.

Normally, any stain that is used for paraffin can be used on GMA  sections,
but you do NOT have to run down to water, as the plastic is in the tissue
forever, it cannot be removed and if you want to do immunostaining, it is
not a good embedding media for that as immunoglobulins cannot penetrate the
plastic matrix to get to the antigens.

Gayle M. Callis
HTl/HT/MT(ASCP)

----- Original Message -----
From: "Suzanne Bruce"  To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Friday, April 25, 2008 4:58 PM
Subject: [Histonet] Staining Procedures for Technovit 7200


Hello!  I'm searching for any staining procedures used in Technovit 7200.
Anyone willing to share some knowledge?

Thanks so much in advance!

Suzanne Bruce,  R.V.T.
Histologist
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