[Histonet] Thioflavin S staining

Tue Apr 22 13:47:23 CDT 2008

Your description gives the impression of damage caused by slow
 freezing tissue, leaving beh buffered formaldehyde, in 30% aqueous sucrose fo sinks). Even with cryoprotection, as quickly as possible. There have been this subject over the years. They can be fou href="http://www.histosearch.com/">www.histos earch.com</A>. &nbsp; John
 Kiernan Anat = = ----- Or "Leskovjan, Andreana" &lt; leskovjan <@t> bnl.gov&gt; Date: Tuesday, April 22,
 2008 staining T histonet <@t> lists.utsouthwestern.edu &gt; Hi, &gt; &gt; &nbsp; &gt;
 & sections with< are mounted onto a film &gt; called Ultralene the best but necessary &gt; measurements.&nbsp; I have been using a ve so &gt; far with &gt; minimal avoid interference with the &gt; measurements: &nbsp; 50% &gt; ethanol for 5 min; 0.0125%
 Thiofl ethanol,then nanopu however &gt; the ti destroyed.&nbsp; There are holes, everywhere, &gt; which makes & plaques difficult to see and measure.&nbsp; I've tried &gt; combinationsof the above and this is the best I c ould get.&nbsp; &gt; Does anyone have
 a &gt; looking &gt; &gt; &nbsp; &gt; &gt; Leskovjan &gt; &gt; _______ _______________________ 5F Histone Histonet <@t> lists.utsouthwestern.edu< http://lists.utsouthwestern.edu/mailman/listinfo/his