[Histonet] ORO

[Hermina Borgerink]

Nearly 25 years ago, we routinely stained gross monkey aortas using Sudan IV, and obtained beautiful results. The only drawback,  a spectrophotometer is required to make up the stain.  I'll be glad to share the protocol in private as it is rather lengthy.

Hermina

Hermina M. Borgerink, BA, HT, HTL(ASCP)QIHC
Wake Forest University Primate Center
Department of Pathology
Medical Center Blvd.
Winston-Salem, NC 27157
Tel. (336) 716-1538
Fax. (336) 716-1515
e-mail:  hborgeri <@t> wfubmc.edu

  

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gayle Callis
Sent: Monday, April 21, 2008 4:43 PM
To: Rene J Buesa; Molinari, Betsy; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] ORO

The Churukian method is not expensive compared to the classic method using propylene glycol (expensive and messy).  A grossly thick cross section of arterial plaque would not be an unreasonable sample to stain with ORO.  If anyone is interested in the Churukian method, published in JOH a few years back, I will be happy to attach privately.

Gayle M. Callis
HTL/HT/MT(ASCP)
Bozeman MT 59715


----- Original Message -----
From: "Rene J Buesa" <rjbuesa <@t> yahoo.com>
To: "Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu>; <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, April 21, 2008 1:58 PM
Subject: Re: [Histonet] ORO


> Sure it can be done, but two things:
>  1- it will be expensive (too much reagent wasted), and
>  2- why would you want to do that?
> René J.
>
> "Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu> wrote:
>  Is it possible to do an ORO on gross samples?
>
> Thanks.
>
>
>
> Betsy Molinari HT(ASCP)
>
> Texas Heart Institute
>
> Cardiovascular Pathology
>
> 6770 Bertner Ave.
>
> MC 1-283
>
> Houston, TX 77030
>
> 832-355-6524
>
> 832-355-6812 (fax)
>
>
>
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