[Histonet] RE: Histonet Digest, Vol 53, Issue 31

[John Spair]

Liz:  Richard Allan has product called Cyto Stain, that and hematoxylin
nuclear stain is all you need for a cyto stain




Date: Sun, 20 Apr 2008 12:52:09 -0600
From: "Liz Chlipala" <liz <@t> premierlab.com>
Subject: [Histonet] need help with PAP stain
To: <histonet <@t> lists.utsouthwestern.edu>
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	<EE33BE5C905A3046A7FF8F58A64C8E4B07FF65 <@t> server.PremierLab.local>
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Hello everyone
 
I need to run a pap stain on some saliva samples and I don't know where
to begin.  I know this seems like a silly question, but I have done some
searching on the net and there are quite a few protocols with different
reagents.  Its not a large project for us, I only need to run about 30
to 40 slides and was hoping to purchase a kit that would contain the
hematoxylin, OG-6 and EA-50 but the only one I can find is the one from
thermo the rapid chrome pap staining kit but I can't seem to find a
vendor that sells it.  If possible I would like to purchase pints of
these reagents rather than gallons. Any advice as to what reagents to
purchase and a protocol would be helpful.  Also advice on what the stain
should look like would help too.
 
Thanks in advance
 
Liz 


"MMS <multicare.org>" made the following annotations.
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