[Histonet] Hepatocyte Specific Antigen

[Sebree Linda A.]

Nancy,

We're using Biocare's HSA (CM 166), 1:50, on our Ventana instruments but
do use HIER.  Off-line we use a high pH Tris buffer for 2" in a lab
pressure cooker and on-line we use Ventana's CC1 which is also a Tris
buffer.  We achieve beautiful, clean staining using these protocols.
Maybe you can adapt this to your Bond stainer.

Good luck,

Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nancy
Schmitt
Sent: Monday, April 21, 2008 11:00 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Hepatocyte Specific Antigen


Hello histonetters - 

Working up Hepatocyte marker on BOND stainer.  Not really getting the
results we wanted.  Calls for no pretreatment - is anybody doing
something
different?

Thanks for your help

Nancy Schmitt MLT, HT (ASCP)
Histology Coordinator
Dubuque, IA


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