[Histonet] immuno controls for cell blocks

[Drew Sally A.]

We are only using routinely processed FFPE tissues as controls for cell
blocks.
Depending on what antibodies you might run on cell blocks, I would think
it difficult and  impractical to try to obtain enough material to create
cell block controls...

Sally Ann Drew, MT(ASCP)
IHC/ISH Laboratory
University of Wisconsin Hosp. & Clinics
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vickroy,
Jim
Sent: Monday, April 21, 2008 8:27 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] immuno controls for cell blocks



We are currently using a bank of formalin fixed tissue controls for our
immunohistochemical stains.   We have done a short study to show that
the reactivity and specificity of our immunostains on the cellblocks,
hardening in 95%ETOH and then transferred in formalin, is not different
than our regularly formalin fixed tissue.
As I read the new CAP checklist this is what we are required to do.
Now a cytopathologist wants me to maintain a set of controls only for
his cellblocks.

How is everybody else handling this situation?

Jim Vickroy
Memorial Medical Center
Springfield, Illinois.




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