[Histonet] ISH protocol

[San Tin]

   Dear All,

   I have some questions with the usage of positive RNA probe in the
   usage of EBV detection. I was told to do positive RNA probe together
   with EBER, kappa and lambda ISH on the test tissue in addition to
   positive controls to make sure that the RNA is properly preserve on
   the fixation provcess.

   Here the problems is do they all need to be the same protocol
   for every probes as my kappa, lambda and EBER are using different
   hybridization temperature and time?


   Please kindly advice.


   San

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References

   1. http://us.rd.yahoo.com/evt=51733/*http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ