[Histonet] IHC Staining Variations

[histobeach <@t> aol.com]

Hello,

We have an automated IHC stainer and the doctors are experiencing what they call, staining variations.? The whole tissue is not staining, just in parts, when areas of the tissues should be staining.? 

Has anyone experienced this problem and if so, what did you do to remedy it?? We were staining manually and used a Pap Pen before we switched to automation and I'm wondering if we are getting areas on the slide that are not being covered with the reagents.? We have 3 drop zones with 100ul of reagent dropping in each zone, for a total of 300ul per slide.

I appreciate any feedback and help.? 

Trinity Whitecomb
Histology Manager
Lab System Works