[Histonet] Snap Freezing of Lymph Nodes for RNA testing

[Andrea Hooper]

Ooops, read this after I already wrote my message 2nding the liquid nitrogen suggestion. 

Well then, based on your comments below I think the RNA Later is ideal. Otherwise you could put the tissue (in small pieces or chopped up) directly into Trizol and ship that way. I have received tissues as such as they have been fine for RNA. But truthfully, RNA Later is probably the safest, easiest way to go.


----- Original Message -----
From: Norm Burnham <Norm.Burnham <@t> propathlab.com>
Date: Wednesday, April 16, 2008 9:31 pm
Subject: Re: [Histonet] Snap Freezing of Lymph Nodes for RNA testing

> Hi Gayle,
> Thank you for your advice. We want to avoid LN and Isopentane if at 
> allpossible.  We are looking for a simple, inexpensive, easy to 
> use, and RNAse
> free method to preserve, stabilize and transport tissue for RNA 
> studies.Does anyone have an alternative to LN or Isopentane bath 
> for snap freezing
> followed by shipment on dry ice, or an alternate way, to stabilize 
> tissue for
> periods ranging from a few hours to perhaps as long as 72 hours?  
> Thanks and best regards,
> Norm Burnham
> 
> ----- Original Message -----
> From: Gayle Callis <gayle.callis <@t> bresnan.net>
> To: Norm Burnham; histonet <@t> lists.utsouthwestern.edu
> <histonet <@t> lists.utsouthwestern.edu>
> Sent: Wed Apr 16 19:20:36 2008
> Subject: Re: [Histonet] Snap Freezing of Lymph Nodes for RNA testing
> 
> It may be better to use a transportable Dewar flask for just the 
> liquid 
> nitrogen.  I don't think a doctors office will want to handle 
> isopentane, 
> too volatile, flammable and explosive.  Contact or maybe those on 
> Histonet 
> in dermatopathology offices have ways to do this for biopsies they 
> collect, 
> freeze and ship.
> 
> Good luck
> 
> Gayle M. Callis
> HTL/HT/MT(ASCP)
> Bozeman MT 59715
> 
> 
> ----- Original Message ----- 
> From: "Norm Burnham" <Norm.Burnham <@t> propathlab.com>
> To: <histonet <@t> lists.utsouthwestern.edu>
> Sent: Wednesday, April 16, 2008 12:50 PM
> Subject: [Histonet] Snap Freezing of Lymph Nodes for RNA testing
> 
> 
> Hi Histonet,
> 
> 
> 
> Does anyone have a simple, inexpensive and very portable method for 
> snapfreezing lymph nodes in preparation for shipment on dry ice?  
> We have
> considered Liquid Nitrogen and Isopentane bath, but I am looking 
> for a fully
> portable method that can be handled in a doctors office by their 
> staff.
> 
> 
> Thanks,
> 
> 
> 
> Norm Burnham
> 
> 
> 
> ____________________________________
> 
> Norm Burnham
> Director of Laboratory Operations
> ProPath - The Leader in Pathology Services(r)
> 
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> Email:  norm.burnham <@t> propath.com <norm.burnham <@t> propath.com>
> 
> 
> To learn more about ProPath, please visit www.ProPath.com
> <http://www.ProPath.com>
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