[Histonet] background of H&E staining

[FU,DONGTAO]

  Thank you all for answering my question. I think I need to 
change our mounting medium first to see whether it helps.

  Have a nice day!

Ann


On Wed Apr 16 07:37:59 EDT 2008, Piero Nelva 
<pieronelva01 <@t> bigpond.com> wrote:

> We had a batch of mounting medium that went yellow, and 
> consequently the section went yellow too (so long ago that I've 
> forgotten the brand).  Since switching to Eukitt, the preparation 
> is much more stable.
> 
> Piero Nelva
> Histology
> Monash Medical Centre
> Australia
> ----- Original Message ----- From: "FU,DONGTAO" <fudo <@t> ufl.edu>
> To: <histonet <@t> lists.utsouthwestern.edu>
> Sent: Wednesday, April 16, 2008 6:21 AM
> Subject: [Histonet] background of H&E staining
> 
> 
>> Hi, all
>> 
>>  Our PI complained about our H&E staining recently. He found the 
>> slides we did for him changed color(within a month), the whole 
>> slides turn to yellow, although the staining is still there and 
>> looks good. I searched for some websites for the answer, but 
>> could not find it.
>> 
>>  Does anyone know the reason why the background of slides 
>> changed color? We did it in the general way which worked well in 
>> the past. And the reagents are not expired. Any suggestions?
>> 
>>  Thanks,
>> 
>> Ann
>> 
>> 
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Ann Dongtao Fu MD, Ph.D
Lab Manager
Dept. of Pathology
Lab phone: 352-273-7752
Lab FAX: 352-273-7755
Lab address: D11-50
PO Box: 100275
1600 SW Archer Road
University of Flodrida
Gainesville, FL 32610