[Histonet] Choosing an antibody

[Gayle Callis]

Hi Liz,

There is a whole set of rat macrophage antibodies, ED1, ED2 and ED3 from 
Serotec and it will depend on which subset one wants to see or as compared 
to a murine F4/80.    We use frozen sections and always a secondary that 
recognizes the mouse IgG isotype from Southern Biotechnology.  These 
antibodies are potent, and we use them at 1:1000 to 1:3000 depending on 
which one is needed.   Be sure to determine working concentration with a 
dilution panel.  I am sure these will work on FFPE tissue but do not have a 
recommendation for retrieval, since we never do that.

They have really interesting and distinct patterns in the rat spleen for 
each subset, fun to do and certainly candidates for some gorgeous double 
staining!

Gayle M. Callis
HTL/HT/MT(ASCP)
Bozeman MT 59715


----- Original Message ----- 
From: "Liz Chlipala" <liz <@t> premierlab.com>
To: "Gayle Callis" <gayle.callis <@t> bresnan.net>
Sent: Monday, April 14, 2008 4:05 PM
Subject: RE: [Histonet] Choosing an antibody


Hey thanks for the update, I did not know that there was one the worked
in rat.

Liz


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 682-3949
fax (303) 682-9060
liz <@t> premierlab.com
www.premierlab.com